Rabbit Polyclonal DNA PKcs antibody. Suitable for IP, WB and reacts with Human samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human PRKDC.
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- Cell death and differentiation 30:1900-19152023DNA-PKcs regulates myogenesis in an Akt-dependent manner independent of induced DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaser Hasan Sutcu,Benjamin Montagne,Miria RicchettiPubMed 37400716
- Journal of translational medicine 21:1832023APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Zhang,Lujie Yang,Han Gao,Xunjie Kuang,He Xiao,Chen Yang,Yi Cheng,Lei Zhang,Xin Guo,Yong Zhong,Mengxia LiPubMed 36894994
- Molecular cancer research : MCR 21:261-2732022ATM Regulation of the Cohesin Complex Is Required for Repression of DNA Replication and Transcription in the Vicinity of DNA Double-Strand Breaks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas E Bass,Donald E Fleenor,Paige E Burrell,Michael B KastanPubMed 36469004
- Cancer research 81:4910-49252021Long Noncoding RNA NIHCOLE Promotes Ligation Efficiency of DNA Double-Strand Breaks in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan P Unfried,Mikel Marín-Baquero,Ángel Rivera-Calzada,Nerea Razquin,Eva M Martín-Cuevas,Sara de Bragança,Clara Aicart-Ramos,Christopher McCoy,Laura Prats-Mari,Raquel Arribas-Bosacoma,Linda Lee,Stefano Caruso,Jessica Zucman-Rossi,Bruno Sangro,Gareth Williams,Fernando Moreno-Herrero,Oscar Llorca,Susan P Lees-Miller,Puri FortesPubMed 34321241
- Frontiers in oncology 11:7005432021Abiraterone Is Superior to Enzalutamide in Response to Ionizing Radiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy C Wright,Victoria L Dunne,Ali H D Alshehri,Kelly M Redmond,Aidan J Cole,Kevin M PrisePubMed 34367984
- mBio 12:2021Herpes Simplex Virus 1 Manipulates Host Cell Antiviral and Proviral DNA Damage Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMax E Mertens,David M KnipePubMed 33563816
- Cell death & disease 12:692021LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Guo et. al.PubMed 33431817
- Aging 12:22174-221982020Nucleolar stress induces a senescence-like phenotype in smooth muscle cells and promotes development of vascular degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Zhang et. al.PubMed 33146634
- Frontiers in oncology 10:5672020MTA1 Promotes Hepatocellular Carcinoma Progression by Downregulation of DNA-PK-Mediated H1.2 Phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Hui Li et. al.PubMed 32435614
- Molecular cancer therapeutics 19:1091-11012020Pharmacologic Inhibitor of DNA-PK, M3814, Potentiates Radiotherapy and Regresses Human Tumors in Mouse Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrank T Zenke et. al.PubMed 32220971
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PRKDC. The exact immunogen used to generate this antibody is proprietary information. Database link P78527
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use at 2 to 5 μg/mg lysate. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage (PubMed:11955432, PubMed:12649176, PubMed:14734805, PubMed:33854234). Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination (PubMed:11955432, PubMed:12649176, PubMed:14734805, PubMed:33854234, PubMed:34352203). Must be bound to DNA to express its catalytic properties (PubMed:11955432). Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C) (PubMed:11955432). Recruited by XRCC5 and XRCC6 to DNA ends and is required to (1) protect and align broken ends of DNA, thereby preventing their degradation, (2) and sequester the DSB for repair by NHEJ (PubMed:11955432, PubMed:12649176, PubMed:14734805, PubMed:15574326, PubMed:33854234). Act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage (PubMed:11955432, PubMed:12649176, PubMed:14734805, PubMed:15574326). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step (PubMed:11955432, PubMed:12649176, PubMed:14734805, PubMed:15574326). Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion (By similarity). Also involved in modulation of transcription (PubMed:11955432, PubMed:12649176, PubMed:14734805, PubMed:15574326). As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome (PubMed:32103174). Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome (PubMed:32103174). Recognizes the substrate consensus sequence [ST]-Q (PubMed:11955432, PubMed:12649176, PubMed:14734805, PubMed:15574326). Phosphorylates 'Ser-139' of histone variant H2AX, thereby regulating DNA damage response mechanism (PubMed:14627815, PubMed:16046194). Phosphorylates ASF1A, DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, FH, SRF, NHEJ1/XLF, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2 (PubMed:10026262, PubMed:10467406, PubMed:11889123, PubMed:12509254, PubMed:14599745, PubMed:14612514, PubMed:14704337, PubMed:15177042, PubMed:1597196, PubMed:16397295, PubMed:18644470, PubMed:2247066, PubMed:2507541, PubMed:26237645, PubMed:26666690, PubMed:28712728, PubMed:29478807, PubMed:30247612, PubMed:8407951, PubMed:8464713, PubMed:9139719, PubMed:9362500). Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA (PubMed:9679063). Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D (PubMed:9363941). Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism (By similarity). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728). Also regulates the cGAS-STING pathway by catalyzing phosphorylation of CGAS, thereby impairing CGAS oligomerization and activation (PubMed:33273464). Also regulates the cGAS-STING pathway by mediating phosphorylation of PARP1 (PubMed:35460603).
Alternative names
HYRC, HYRC1, PRKDC, DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNPK1, p460
Recommended products
Rabbit Polyclonal DNA PKcs antibody. Suitable for IP, WB and reacts with Human samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human PRKDC.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PRKDC. The exact immunogen used to generate this antibody is proprietary information. Database link P78527
- Purification technique
- Affinity purification Immunogen
- Specificity
Ku-80 co-immunoprecipitates using this antibody.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DNA-dependent protein kinase catalytic subunit often abbreviated as DNA-PKcs or PKcs is an important component in the cellular machinery responsible for DNA repair. This protein weighs approximately 470 kDa and is predominantly expressed in various tissue types with higher levels found in lymphoid tissues. The DNA-PKcs functions as a serine/threonine protein kinase and becomes active upon binding to DNA contributing to its role in maintaining genome integrity.
- Biological function summary
DNA-PKcs plays an essential role in the non-homologous end joining (NHEJ) repair pathway which repairs double-strand breaks in DNA. DNA-PKcs forms a complex with the Ku70/80 heterodimer serving as a critical component of the DNA repair mechanism. This complex recognizes DNA ends facilitates their synapsis and activates other enzymes involved in ligating the broken DNA strands therefore promoting cellular survival following DNA damage.
- Pathways
DNA-PKcs deeply integrates into the DNA damage response pathway. It collaborates closely with proteins such as ATM (ataxia-telangiectasia mutated) to sense DNA damage and initiate repair. DNA-PKcs is involved in V(D)J recombination vital for the generation of diverse antibodies in immune cells. Its activity is essential for coordinating with other proteins including XRCC4 and Ligase IV to ensure efficient DNA repair processes are executed.
- Associated diseases and disorders
Defects in DNA-PKcs are linked to severe combined immunodeficiency (SCID) due to its central role in V(D)J recombination. Dysfunction in its pathway also relates to cancer where impaired DNA repair mechanisms substantially increase genomic instability leading to tumorigenesis. DNA-PKcs interacts with other proteins like BRCA1 and p53 in tumors where its overexpression or mutations can contribute to resistance against radiation and chemotherapeutic agents. These interactions highlight the importance of DNA-PK inhibitors as potential therapeutic strategies in oncology.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunoprecipitation - Anti-DNA PKcs antibody (ab70250)
Immunoprecipitation/ Western Blot of DNA PKcs
Lane 1: ab70250 at 3μg/100μg for IP of HeLa whole cell lysate (1mg) followed by ab70250 at 0.1μg/ml for WB.
Lane 2: Control IgG.
Chemiluminescence with an exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-DNA PKcs antibody (ab70250)
Predicted band size: 469 kDa
Western blot - Anti-DNA PKcs antibody (ab70250)
All lanes: Western blot - Anti-DNA PKcs antibody (ab70250) at 0.1 µg/mL
Lane 1: Whole cell lysate from Hela cells at 15 µg
Lane 2: Whole cell lysate from Hela cells at 50 µg
Predicted band size: 469 kDa
Observed band size: 170 kDa, 180 kDa, 230 kDa, 469 kDa
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