AB176734

Anti-DNA polymerase alpha/POLA antibody

Name: Anti-DNA polymerase alpha/POLA antibody (ab176734) | Abcam
Brand: Abcam Limited
SKU: AB176734

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(2 Publications)

Rabbit Polyclonal DNA polymerase alpha/POLA antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human POLA1 aa 1150-1250.

View Alternative Names

POLA, POLA1, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180

2 Images

Supplier Data

Immunoprecipitation - Anti-DNA polymerase alpha/POLA antibody (AB176734)

ab176734 at 0.4 μg/ml detecting DNA polymerase alpha/POLA in HeLa whole cell lysate by WB following IP. ab176734 was used for IP at 3 μg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-DNA polymerase alpha/POLA antibody (ab176734)

Predicted band size: 166 kDa

false

Supplier Data

Western blot - Anti-DNA polymerase alpha/POLA antibody (AB176734)

All lanes:

Western blot - Anti-DNA polymerase alpha/POLA antibody (ab176734) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 166 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human POLA1 aa 1150-1250. The exact immunogen used to generate this antibody is proprietary information.

P09884

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176734 is affinity purified using an epitope specific to DNA polymerase alpha/POLA immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, a regulatory subunit POLA2 and two primase subunits PRIM1 and PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA : DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed : 27019227).

See full target information POLA1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:2426 PubMed31160578

2019

The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing.

Applications

Unspecified application

Species

Unspecified reactive species

Philippe Coulombe,Joelle Nassar,Isabelle Peiffer,Slavica Stanojcic,Yvon Sterkers,Axel Delamarre,Stéphane Bocquet,Marcel Méchali

Nature communications 8:1392 PubMed29123096

2017

ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Moiseeva,Brian Hood,Sandy Schamus,Mark J O'Connor,Thomas P Conrads,Christopher J Bakkenist

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNA polymerase alpha/POLA antibody

Immunoprecipitation - Anti-DNA polymerase alpha/POLA antibody (AB176734)

Western blot - Anti-DNA polymerase alpha/POLA antibody (AB176734)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise