AB31777

Anti-DNA polymerase alpha/POLA antibody

Name: Anti-DNA polymerase alpha/POLA antibody (ab31777) | Abcam
Brand: Abcam Limited
SKU: AB31777
Price: 510 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(12 Publications)

Rabbit Polyclonal DNA polymerase alpha/POLA antibody. Suitable for WB, IHC-P and reacts with Human, Xenopus laevis, Chicken samples. Cited in 12 publications.

View Alternative Names

POLA, POLA1, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180

1 Images

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Western blot - Anti-DNA polymerase alpha/POLA antibody (AB31777)

All lanes:

Western blot - Anti-DNA polymerase alpha/POLA antibody (ab31777) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - Jurkat whole cell lysate (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 20 µg

Lane 3:

Western blot - A-431 whole cell lysate (<a href='/en-us/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Predicted band size: 166 kDa

Observed band size: 180 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Xenopus laevis, Chicken

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, a regulatory subunit POLA2 and two primase subunits PRIM1 and PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA : DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed : 27019227).

See full target information POLA1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37762371

2023

The Antitumor Effect of the DNA Polymerase Alpha Inhibitor ST1926 in Glioblastoma: A Proteomics Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Chirine El-Baba,Zeinab Ayache,Mona Goli,Berthe Hayar,Zeinab Kawtharani,Claudio Pisano,Firas Kobeissy,Yehia Mechref,Nadine Darwiche

Nucleic acids research 49:11653-11665 PubMed34718732

2021

CST does not evict elongating telomerase but prevents initiation by ssDNA binding.

Applications

Unspecified application

Species

Unspecified reactive species

Arthur J Zaug,Ci Ji Lim,Conner L Olson,Maria T Carilli,Karen J Goodrich,Deborah S Wuttke,Thomas R Cech

Molecular cell 80:682-698.e7 PubMed33152268

2020

Hyperactive CDK2 Activity in Basal-like Breast Cancer Imposes a Genome Integrity Liability that Can Be Exploited by Targeting DNA Polymerase ε.

Applications

Unspecified application

Species

Unspecified reactive species

Vladislav O Sviderskiy,Lili Blumenberg,Elizabeth Gorodetsky,Triantafyllia R Karakousi,Nicole Hirsh,Samantha W Alvarez,Erdem M Terzi,Efiyenia Kaparos,Gabrielle C Whiten,Shakirah Ssebyala,Peter Tonzi,Hannan Mir,Benjamin G Neel,Tony T Huang,Sylvia Adams,Kelly V Ruggles,Richard Possemato

The Journal of biological chemistry 295:15029-15044 PubMed32839273

2020

Implications of RNG140 (caprin2)-mediated translational regulation in eye lens differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Kaori Nakazawa,Yuichi Shichino,Shintaro Iwasaki,Nobuyuki Shiina

Cell reports 30:2416-2429.e7 PubMed32075739

2020

Physiological Tolerance to ssDNA Enables Strand Uncoupling during DNA Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Amaia Ercilla,Jan Benada,Sampath Amitash,Gijs Zonderland,Giorgio Baldi,Kumar Somyajit,Fena Ochs,Vincenzo Costanzo,Jiri Lukas,Luis Toledo

Molecular cell 70:707-721.e7 PubMed29754823

2018

Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Bellelli,Valerie Borel,Clare Logan,Jennifer Svendsen,Danielle E Cox,Emma Nye,Kay Metcalfe,Susan M O'Connell,Gordon Stamp,Helen R Flynn,Ambrosius P Snijders,François Lassailly,Andrew Jackson,Simon J Boulton

American journal of cancer research 8:39-55 PubMed29416919

2018

Mechanism of action of the atypical retinoid ST1926 in colorectal cancer: DNA damage and DNA polymerase α.

Applications

Unspecified application

Species

Unspecified reactive species

Rana Abdel-Samad,Patrick Aouad,Hala Gali-Muhtasib,Zeinab Sweidan,Raed Hmadi,Humam Kadara,Egildo Luca D'Andrea,Alessandra Fucci,Claudio Pisano,Nadine Darwiche

Nature communications 8:2130 PubMed29242643

2017

RNAs coordinate nuclear envelope assembly and DNA replication through ELYS recruitment to chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Aze,Michalis Fragkos,Stéphane Bocquet,Julien Cau,Marcel Méchali

Molecular cell 67:867-881.e7 PubMed28757209

2017

Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments.

Applications

Unspecified application

Species

Unspecified reactive species

Arun Mouli Kolinjivadi,Vincenzo Sannino,Anna De Antoni,Karina Zadorozhny,Mairi Kilkenny,Hervé Técher,Giorgio Baldi,Rong Shen,Alberto Ciccia,Luca Pellegrini,Lumir Krejci,Vincenzo Costanzo

Nucleic acids research 44:558-72 PubMed26420833

2015

Reduced cohesin destabilizes high-level gene amplification by disrupting pre-replication complex bindings in human cancers with chromosomal instability.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyeon Yun,Sang-Hyun Song,Jee-Youn Kang,Jinah Park,Hwang-Phill Kim,Sae-Won Han,Tae-You Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNA polymerase alpha/POLA antibody

Western blot - Anti-DNA polymerase alpha/POLA antibody (AB31777)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (12)

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