Anti-DNA Polymerase beta antibody [EPR12722(B)]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (AB175197)

Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling DNA Polymerase beta with Purified ab175197 at 1 : 50 dilution (3.1 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (AB175197)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling with Purified ab175197 at 1 : 500 dilution (0.31 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. M3

• Flow Cyt

Lab

Flow Cytometry - Anti-DNA Polymerase beta antibody [EPR12722(B)] (AB175197)

Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labelling DNA Polymerase beta with Purified ab175197 at 1 : 200 dilution (1 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (AB175197)

Immunocytochemistry analysis of Neuro-2a (mouse neuroblastoma neuroblast) cells labeling DNA Polymerase beta with ab175197 at 1/50 dilution (1.57 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Confocal image showing nuclear staining in Neuro-2a cell line (shown in green). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• WB

Unknown

Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (AB175197)

All lanes:

Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 3:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 4:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Mouse brain lysate at 20 µg

Lane 6:

Mouse spleen lysate at 20 µg

Lane 7:

Rat brain lysate at 20 µg

Lane 8:

Rat spleen lysate

Lane 9:

C6 (Rat glial tumor glial cell) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

false

• WB

CiteAb

Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (AB175197)

DNA Polymerase beta western blot using anti-DNA Polymerase beta antibody [EPR12722(B)] ab175197. Publication image and figure legend from Wang, H., Chen, Y., et al., 2014, PLoS One, PubMed 25303312.

ab175197 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab175197 please see the product overview.

DNA poly β, cyclin E and cyclin D expression were detected after treatment with low and high concentrations of rotenone.The expression of DNA poly β, cyclin E and cyclin D were investigated by western blot. * P<0.05 compared to the control group; # P>0.05 compared to the control group.

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