Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (AB249852)

This data was developed using ab175197, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling DNA Polymerase beta with Purified ab175197 at 1 : 50 dilution (3.1 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (AB249852)

This data was developed using ab249852, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling DNA Polymerase beta with Purified ab249852 at 1 : 500 dilution (0.31 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. M3

• Flow Cyt

Lab

Flow Cytometry - Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (AB249852)

This data was developed using ab249852, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labelling DNA Polymerase beta with Purified ab249852 at 1 : 200 dilution (1 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (AB249852)

This data was developed using ab175197, the same antibody clone in a different buffer formulation. Immunocytochemistry analysis of Neuro-2a (mouse neuroblastoma neuroblast) cells labeling DNA Polymerase beta with ab175197 at 1/50 dilution (1.57 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Confocal image showing nuclear staining in Neuro-2a cell line (shown in green). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• WB

Unknown

Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (AB249852)

All lanes:

Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (<a href='/en-us/products/primary-antibodies/dna-polymerase-beta-antibody-epr12722b-ab175197'>ab175197</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 3:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 4:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Mouse brain lysate at 20 µg

Lane 6:

Mouse spleen lysate at 20 µg

Lane 7:

Rat brain lysate at 20 µg

Lane 8:

Rat spleen lysate

Lane 9:

C6 (Rat glial tumor glial cell) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

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