Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free

5 product images

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  Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (ab249852)

  All lanes: Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (Anti-DNA Polymerase beta antibody [EPR12722(B)] ab175197) at 1/1000 dilution

  Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

  Lane 3: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

  Lane 4: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 5: Mouse brain lysate at 20 µg

  Lane 6: Mouse spleen lysate at 20 µg

  Lane 7: Rat brain lysate at 20 µg

  Lane 8: Rat spleen lysate

  Lane 9: C6 (Rat glial tumor glial cell) whole cell lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 38 kDa

  Observed band size: 38 kDa

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  Flow Cytometry - Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (ab249852)

  This data was developed using ab249852, the same antibody clone in a different buffer formulation.
  Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labelling DNA Polymerase beta with Purified ab249852 at 1:200 dilution (1 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (ab249852)

  This data was developed using ab249852, the same antibody clone in a different buffer formulation.
  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling DNA Polymerase beta with Purified ab249852 at 1:500 dilution (0.31 ?g/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. M3

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  Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (ab249852)

  This data was developed using Anti-DNA Polymerase beta antibody [EPR12722(B)] ab175197, the same antibody clone in a different buffer formulation.
  Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling DNA Polymerase beta with Purified Anti-DNA Polymerase beta antibody [EPR12722(B)] ab175197 at 1:50 dilution (3.1 ?g/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (ab249852)

  This data was developed using Anti-DNA Polymerase beta antibody [EPR12722(B)] ab175197, the same antibody clone in a different buffer formulation.
  
  Immunocytochemistry analysis of Neuro-2a (mouse neuroblastoma neuroblast) cells labeling DNA Polymerase beta with Anti-DNA Polymerase beta antibody [EPR12722(B)] ab175197 at 1/50 dilution (1.57 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. PBS instead of the primary antibody was used as the secondary antibody only control.
  
  Confocal image showing nuclear staining in Neuro-2a cell line (shown in green). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).