JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308159

Anti-DNA polymerase beta antibody [HL1512]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal DNA Polymerase beta antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human POLB.

View Alternative Names

DNA polymerase beta, 5'-deoxyribose-phosphate lyase, AP lyase, 5'-dRP lyase, POLB

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase beta antibody [HL1512] (AB308159)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase beta antibody [HL1512] (AB308159)

For immunofluorescence analysis, HeLa cells were fixed in ice-cold MeOH for 10 min and permeabilized for detection of DNA polymerase beta using ab308159 at 1/250 (green)

Alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1/1000 (red)

Nucleus was stained with Fluoroshield with DAPI (blue)

Scale bar= 10μm

Immunohistochemistry paraffin embedded sections - Anti-DNA polymerase beta antibody [HL1512] (AB308159)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-DNA polymerase beta antibody [HL1512] (AB308159)

Immunohistochemical analysis of Paraffin-embedded rat testis tissue labeling DNA polymerase beta with ab308159 at 1/100 dilution. Heat mediated antigen retrieval with citrate buffer pH 6.0 for 15 mins.

Immunohistochemistry paraffin embedded sections - Anti-DNA polymerase beta antibody [HL1512] (AB308159)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-DNA polymerase beta antibody [HL1512] (AB308159)

Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue labeling DNA polymerase beta with ab308159 at 1/100 dilution. Heat mediated antigen retrieval with citrate buffer pH 6.0 for 15 mins.

Western blot - Anti-DNA polymerase beta antibody [HL1512] (AB308159)
  • WB

Supplier Data

Western blot - Anti-DNA polymerase beta antibody [HL1512] (AB308159)

The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-DNA polymerase beta antibody [HL1512] (ab308159) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

POLB knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 38 kDa

false

Western blot - Anti-DNA polymerase beta antibody [HL1512] (AB308159)
  • WB

Supplier Data

Western blot - Anti-DNA polymerase beta antibody [HL1512] (AB308159)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (ab308159) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-DNA polymerase beta antibody [HL1512] (ab308159) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

HepG2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 38.2 kDa

false

Western blot - Anti-DNA polymerase beta antibody [HL1512] (AB308159)
  • WB

Supplier Data

Western blot - Anti-DNA polymerase beta antibody [HL1512] (AB308159)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (ab308159) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-DNA polymerase beta antibody [HL1512] (ab308159) at 1/1000 dilution

Lane 1:

Neuro2a cell lysate at 30 µg

Lane 2:

Rat2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 38.2 kDa

false

Western blot - Anti-DNA polymerase beta antibody [HL1512] (AB308159)
  • WB

Supplier Data

Western blot - Anti-DNA polymerase beta antibody [HL1512] (AB308159)

Separated by 10% SDS-PAGE.

Lane 1:

Western blot - Anti-DNA polymerase beta antibody [HL1512] (ab308159) at 1/1000 dilution

Lane 1:

Whole zebrafish lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 38.2 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1512

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat, Zebrafish

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human POLB. The exact immunogen used to generate this antibody is proprietary information.

P06746

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DNA polymerase beta often abbreviated as pol β serves as a repair enzyme that plays a role in the base excision repair (BER) pathway. This protein's mechanical function involves filling small gaps in the DNA created during repair processes by adding nucleotides. Its molecular weight is approximately 39 kDa. DNA polymerase beta is expressed extensively in the brain liver and testis indicating its importance in DNA repair in various cells. Other names for this protein include DNA pol beta and pol β. It synthesizes DNA using deoxyribonucleotide triphosphates as substrates and requires magnesium ions for activity.
Biological function summary

DNA polymerase beta helps to maintain genome integrity by repairing DNA lesions caused by oxidation alkylation or deamination. This protein often collaborates with other components of the DNA repair pathways but it does not directly form a larger protein complex focusing instead on its important function in BER. When DNA damage like single-strand breaks occur pol β carries out important gap-filling synthesis steps preparing the strand for subsequent ligation.

Pathways

DNA polymerase beta functions critically in base excision repair and acts alongside other important proteins like XRCC1. The BER pathway operates to correct DNA damage from endogenous sources preventing harmful mutations. This pathway is essential for maintaining cellular stability and protecting cells from apoptosis or malignant transformation that might occur if DNA damage propagates.

Mutations or dysfunctional activity within DNA polymerase beta have links to cancer and neurodegenerative diseases. For example errors in pol β's repair functions can lead to the persistence of DNA damage which can contribute to tumorigenesis. Furthermore its relationship with beta-actin has implications in cellular structural integrity and cancer metastasis. Dysfunctions within the BER pathway indicate potential contributions to neurodegenerative disorders where an accumulation of DNA damage is a common feature.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Repair polymerase that plays a key role in base-excision repair (PubMed : 10556592, PubMed : 9207062, PubMed : 9572863). During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap (PubMed : 10556592, PubMed : 17526740, PubMed : 9556598, PubMed : 9572863, PubMed : 9614142). Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase (PubMed : 9614142).
See full target information POLB
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB134056

Anti-XRCC1 antibody [EPR4389(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)

  • 5
  • 3
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB313375

Anti-DNA Ligase III/LIG3 antibody [EPR27265-44] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DNA Ligase III/LIG3 antibody [EPR27265-44] - BSA and Azide free (AB313375)

  • 0
  • 0
Primary Antibodies

AB81299

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB81299)

  • 4
  • 17

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com