AB236450

Anti-DNA polymerase eta antibody

Name: Anti-DNA polymerase eta antibody (ab236450) | Abcam
Brand: Abcam Limited
SKU: AB236450
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal DNA polymerase eta antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POLH.

View Alternative Names

RAD30, RAD30A, XPV, POLH, DNA polymerase eta, RAD30 homolog A, Xeroderma pigmentosum variant type protein

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase eta antibody (AB236450)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase eta antibody (AB236450)

U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DNA polymerase eta (green) using ab236450 at 1/2000 dilution in ICC/IF (green). The nuclear counter stain is DAPI (blue). The right-hand panel shows the merged images.

Flow Cytometry - Anti-DNA polymerase eta antibody (AB236450)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-DNA polymerase eta antibody (AB236450)

Flow cytometric analysis of 1% PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cell line labeling DNA polymerase eta with ab236450 at 1/200 dilution (green) compared with a no primary antibody control (blue).

Supplier Data

Western blot - Anti-DNA polymerase eta antibody (AB236450)

Blocking buffer : 5% milk in TBS-T

Incubated overnight with primary antibody.

All lanes:

Western blot - Anti-DNA polymerase eta antibody (ab236450) at 1/2000 dilution

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell line) treated with control siRNA, whole cell lysate at 40 µg

Lane 2:

U-2 OS treated with DNA polymerase eta siRNA, whole cell lysate at 40 µg

Lane 3:

MEF (mouse embryonic fibroblast cell line) whole cell lysate at 40 µg

Lane 4:

INS-1 (rat pancreatic beta cell line) whole cell lysate at 40 µg

Predicted band size: 78 kDa

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Supplier Data

Immunoprecipitation - Anti-DNA polymerase eta antibody (AB236450)

DNA polymerase eta was immunoprecipitated from ~400 μg U-2 OS (human bone osteosarcoma epithelial cell line) whole cell extract with 3 μl ab236450. Western blot was performed from the immunoprecipitate using ab236450 at 1/2000 dilution.

Lane 1 : U-2 OS whole cell extract (Input)

Lane 2 : Control IgG IP in U-2 OS whole cell extract.

Lane 3 : ab236450 IP in U-2 OS whole cell extract.

All lanes:

Immunoprecipitation - Anti-DNA polymerase eta antibody (ab236450)

Predicted band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP, ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human POLH. The exact immunogen used to generate this antibody is proprietary information.

Q9Y253

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 3 μl to ~400 μg of extact.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed : 10385124, PubMed : 11743006, PubMed : 16357261, PubMed : 24449906, PubMed : 24553286, PubMed : 38212351). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed : 24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed : 10385124, PubMed : 11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed : 11376341, PubMed : 14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed : 14630940). Targets POLI to replication foci (PubMed : 12606586).

See full target information POLH

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 182:3594-3611 PubMed40194519

2025

Inhibition of DNA polymerase eta-mediated translesion DNA synthesis with small molecule sensitises ovarian cancer stem-like cells to chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Subhankar Bose,Priyanka Saha,Md Tanjim Alam,Bilash Chatterjee,Mrinmoy Sarkar,Amit Kumar Dixit,Deepak Kumar,Rakesh Kumar Pathak,Prem Prakash Tripathi,Amit Kumar Srivastava

Genomics, proteomics & bioinformatics 20:597-613 PubMed33607295

2021

Phosphoproteomics Reveals the AMPK Substrate Network in Response to DNA Damage and Histone Acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuejing Jiang,Xiaoji Cong,Shangwen Jiang,Ying Dong,Lei Zhao,Yi Zang,Minjia Tan,Jia Li

Cell reports 31:107542 PubMed32375027

2020

Systematic Analysis of Targets of Pumilio-Mediated mRNA Decay Reveals that PUM1 Repression by DNA Damage Activates Translesion Synthesis.

Applications

WB, IF

Species

Unspecified reactive species, Unspecified reactive species

Toshimichi Yamada,Naoto Imamachi,Katsutoshi Imamura,Kenzui Taniue,Takeshi Kawamura,Yutaka Suzuki,Masami Nagahama,Nobuyoshi Akimitsu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNA polymerase eta antibody

Immunocytochemistry/ Immunofluorescence - Anti-DNA polymerase eta antibody (AB236450)

Flow Cytometry - Anti-DNA polymerase eta antibody (AB236450)

Western blot - Anti-DNA polymerase eta antibody (AB236450)

Immunoprecipitation - Anti-DNA polymerase eta antibody (AB236450)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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