Rabbit Polyclonal DNA polymerase I antibody. Suitable for IP, WB and reacts with Escherichia coli samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Escherichia coli K-12 polA.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.05% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Escherichia coli K-12 polA. Database link P00582
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Escherichia coli | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.
Additional Targets
DNA polymerase I
Alternative names
resA, b3863, JW3835, polA, DNA polymerase I, POL I
Recommended products
Rabbit Polyclonal DNA polymerase I antibody. Suitable for IP, WB and reacts with Escherichia coli samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Escherichia coli K-12 polA.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.05% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Escherichia coli K-12 polA. Database link P00582
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-DNA polymerase I antibody (ab188424)
All lanes: Western blot - Anti-DNA polymerase I antibody (ab188424) at 1/2000 dilution
Lane 1: Purified Escherichia coli DNA polymerase alpha at 0.01 µg
Lane 2: Sonic lysate supernatant of Escherichia coli strain ab1157 cells
Predicted band size: 103 kDa
Immunoprecipitation - Anti-DNA polymerase I antibody (ab188424)
ab188424 detecting DNA polymerase alpha in Escherichia coli strain ab1157 crude cell lysate was precipitated with protein G conjugated magnetic beads and analyzed by WB by using ab188424 antibody.
ab 131366 VeriBlot for IP secondary antibody (HRP) was used for secondary staining.All lanes: Immunoprecipitation - Anti-DNA polymerase I antibody (ab188424)
Downloads
Product protocols
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