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AB188424

Anti-DNA polymerase I antibody

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(2 Publications)

Rabbit Polyclonal DNA polymerase I antibody. Suitable for IP, WB and reacts with Escherichia coli samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Escherichia coli K-12 polA.

View Alternative Names

resA, b3863, JW3835, polA, DNA polymerase I, POL I

2 Images
Immunoprecipitation - Anti-DNA polymerase I antibody (AB188424)
  • IP

Supplier Data

Immunoprecipitation - Anti-DNA polymerase I antibody (AB188424)

ab188424 detecting DNA polymerase alpha in Escherichia coli strain ab1157 crude cell lysate was precipitated with protein G conjugated magnetic beads and analyzed by WB by using ab188424 antibody.
ab 131366 VeriBlot for IP secondary antibody (HRP) was used for secondary staining.

All lanes:

Immunoprecipitation - Anti-DNA polymerase I antibody (ab188424)

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Western blot - Anti-DNA polymerase I antibody (AB188424)
  • WB

Supplier Data

Western blot - Anti-DNA polymerase I antibody (AB188424)

All lanes:

Western blot - Anti-DNA polymerase I antibody (ab188424) at 1/2000 dilution

Lane 1:

Purified Escherichia coli DNA polymerase alpha at 0.01 µg

Lane 2:

Sonic lysate supernatant of Escherichia coli strain ab1157 cells

Predicted band size: 103 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Escherichia coli

Applications

IP, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Escherichia coli K-12 polA.

P00582

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.
See full target information polA

Additional targets

DNA polymerase I
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40631979

2025

Fast evolution of SOS-independent multi-drug resistance in bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Le Zhang,Yunpeng Guan,YuenYee Cheng,Nural N Cokcetin,Amy L Bottomley,Andrew Robinson,Elizabeth J Harry,Antoine M van Oijen,Qian Peter Su,Dayong Jin

Nature communications 16:5342 PubMed40533478

2025

Reversible proliferative arrest induced by rapid depletion of RNase MRP.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Liu,Shiyang He,Kawon Pyo,Reuben Franklin,Ibrahim B Maaz,Chen Cai,Kriti Shah,Sihem Cheloufi,William F Marzluff,Jernej Murn
View all publications
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Product promise

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