AB264193

Anti-DNA Polymerase iota antibody

Name: Anti-DNA Polymerase iota antibody (ab264193) | Abcam
Brand: Abcam Limited
SKU: AB264193

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Polyclonal DNA Polymerase iota antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human POLI aa 650 to C-terminus.

View Alternative Names

RAD30B, POLI, DNA polymerase iota, Eta2, RAD30 homolog B

2 Images

Supplier Data

Immunoprecipitation - Anti-DNA Polymerase iota antibody (AB264193)

DNA Polymerase iota was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264193 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab264193 at 1 μg/ml.
Lane 1 : ab264193 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-DNA Polymerase iota antibody (ab264193)

Predicted band size: 83 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-DNA Polymerase iota antibody (AB264193)

4-8% SDS-PAGE.

Secondary : Goat anti-Rabbit Light Chain HRP Conjugate.

All lanes:

Western blot - Anti-DNA Polymerase iota antibody (ab264193) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T whole cell lysate at 50 µg

Predicted band size: 83 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human POLI aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UNA4

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DNA Polymerase iota also known as POLI is a specialized DNA polymerase involved in DNA repair processes. It is characterized by its ability to catalyze translesion synthesis permitting DNA replication across lesions that stall the replication fork. Mechanically POLI incorporates nucleotides opposite certain types of DNA damage albeit with higher error rates compared to other polymerases. It is encoded by the POLI gene which in humans is located on chromosome 18. The mass of the protein approximates 83kDa. DNA Polymerase iota is expressed primarily in the testis and is found in other tissues but in lower amounts. Its localization and activity underline its unique roles in maintaining genome stability under conditions that challenge the DNA replication machinery.

Biological function summary

DNA Polymerase iota participates in the repair of DNA through its function in translesion synthesis allowing cells to replicate their DNA in the presence of specific types of damage. It operates independently and it does not form a stable complex like some other polymerases such as those in the DNA polymerase zeta pathway. Its incorporation of nucleotides can lead to mutations due to its lower fidelity which can be both beneficial in tolerating damage and risky due to potential mutagenesis. This trade-off is critical for its biological role particularly during spermatogenesis where rapid proliferation occurs.

Pathways

DNA Polymerase iota is an important player in the DNA damage tolerance pathway specifically in the translesion synthesis process. It functions in coordination with other members of the Y-family of DNA polymerases including DNA Polymerase eta (POLH) and kappa (POLK) which contribute to the bypassing of DNA lesions. These interactions allow cells to survive DNA damage that is beyond immediate repair and continue replication though it may introduce mutations. POLI's activities are vital in the damage bypass pathway that complements the nucleotide excision repair process.

DNA Polymerase iota links to an increased risk of certain cancers due to its error-prone DNA synthesis. Its activity may lead to mutations that drive carcinogenesis when coupled with other genetic mutations or environmental factors. It has significant relevance in conditions related to genomic instability such as skin cancer in relation to ultraviolet-induced DNA damage. Moreover POLI’s role correlates with XPC a DNA damage recognition protein in disorders such as Xeroderma pigmentosum. Dysfunction in these pathways that involve POLI highlights its engagement in maintaining genomic integrity and the balance between DNA repair accuracy and tolerance of lesion persistence.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Error-prone DNA polymerase specifically involved in DNA repair (PubMed : 11013228, PubMed : 11387224). Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed : 11013228, PubMed : 11387224, PubMed : 14630940, PubMed : 15199127). Favors Hoogsteen base-pairing in the active site (PubMed : 15254543). Inserts the correct base with high-fidelity opposite an adenosine template (PubMed : 15254543). Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine (PubMed : 11013228). May play a role in hypermutation of immunoglobulin genes (PubMed : 12410315). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (PubMed : 11251121, PubMed : 14630940).

See full target information POLI

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNA Polymerase iota antibody

Immunoprecipitation - Anti-DNA Polymerase iota antibody (AB264193)

Western blot - Anti-DNA Polymerase iota antibody (AB264193)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise