JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236985

Anti-DNA Polymerase lambda antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal DNA Polymerase lambda/Polk antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human POLL aa 1-250.

View Alternative Names

DNA polymerase lambda, Pol Lambda, DNA polymerase beta-2, DNA polymerase kappa, Pol beta2, POLL

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase lambda antibody (AB236985)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase lambda antibody (AB236985)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for DNA Polymerase lambda/Polk (green) using ab236985 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human POLL aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UGP5

style
left-column

Alternative Products

Primary Antibodies

AB172974

Anti-DNA Polymerase lambda antibody [EPR7519(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (AB172974)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB269855

Anti-IGJ antibody [EPR23130-113]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] (AB269855)

  • 0
  • 0
Primary Antibodies

AB157465

Anti-DNA polymerase mu antibody [EPR10470(B)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (AB157465)

  • 0
  • 0
Primary Antibodies

AB86076

Anti-DNA Polymerase Kappa/POLK antibody

Western blot - Anti-DNA Polymerase Kappa/POLK antibody (AB86076)

  • 0
  • 0
Primary Antibodies

AB31777

Anti-DNA polymerase alpha/POLA antibody

Western blot - Anti-DNA polymerase alpha/POLA antibody (AB31777)

  • 4
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DNA polymerase lambda also known as pol λ or DNA pol λ is a DNA polymerase protein involved in DNA repair and replication processes. It has a molecular weight of approximately 65 kDa. DNA polymerase lambda is expressed in various tissues but shows a high concentration in testis and thymus indicating its role in germ cells and immune response. The enzyme exhibits both DNA polymerase and terminal transferase activities facilitating DNA synthesis across gaps and lesions.
Biological function summary

DNA polymerase lambda acts as a gap-filling enzyme during DNA repair processes especially in base excision repair and non-homologous end joining. This polymerase does not typically function within a larger complex but requires accessory factors to perform its repair functions. It maintains genomic stability by providing an accurate extension of DNA strands during repair ensuring the correct rejoining of DNA. Furthermore it also exhibits a lyase activity that can remove 5'-deoxyribose phosphate residues.

Pathways

DNA polymerase lambda is actively involved in the base excision repair and non-homologous end joining pathways. These pathways are important for fixing DNA single-strand breaks and double-strand breaks respectively. Pol λ works alongside proteins like XRCC1 in the short-patch base excision repair pathway and interacts with KU70/80 in non-homologous end joining reinforcing its multifaceted role in maintaining DNA integrity under varying genotoxic stress conditions.

DNA polymerase lambda is linked to conditions such as cancer and immunodeficiencies where its dysfunction can lead to genomic instability. The loss or aberrant function of DNA pol λ may result in ineffective DNA repair contributing to cancer development due to accumulated DNA damage. It can also interact with tumor suppressor p53 highlighting its involvement in pathways that prevent oncogenesis. Additionally impairments in its function can contribute to immunodeficiency disorders as proper DNA repair is essential for the development and function of the immune system.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

DNA polymerase that functions in several pathways of DNA repair (PubMed : 11457865, PubMed : 19806195, PubMed : 20693240, PubMed : 30250067). Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PubMed : 11457865, PubMed : 19806195). Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination (PubMed : 19806195, PubMed : 20693240, PubMed : 30250067). Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities (PubMed : 10887191, PubMed : 10982892, PubMed : 12809503, PubMed : 14627824, PubMed : 15537631, PubMed : 19806195). Also has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity (PubMed : 11457865, PubMed : 19806195).
See full target information POLL
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109675 PubMed38706837

2024

extract attenuates corticotropin-releasing hormone-induced cellular senescence in human hair follicle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sunhyoung Lee,So Young Kim,Seunghee Lee,Sunhyae Jang,Sungjoo Tommy Hwang,Youngji Kwon,Jaehwan Choi,Ohsang Kwon
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com