AB249743

Anti-DNA Polymerase lambda antibody [EPR7519(2)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal DNA Polymerase lambda/Polk antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

DNA polymerase lambda, Pol Lambda, DNA polymerase beta-2, DNA polymerase kappa, Pol beta2, POLL

1 Images

Lab

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] - BSA and Azide free (AB249743)

This data was developed using the same antibody clone in a different buffer formulation (ab172974).

Lanes 1-3 : Merged signal (red and green). Green - ab172974 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab172974 Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] was shown to specifically react with DNA Polymerase lambda/Polk in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264754 (knockout cell lysate ab258595) was used. Wild-type and DNA Polymerase lambda/Polk knockout samples were subjected to SDS-PAGE. ab172974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (<a href='/en-us/products/primary-antibodies/dna-polymerase-lambda-antibody-epr75192-ab172974'>ab172974</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

POLL knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human POLL (DNA Polymerase lambda) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-poll-dna-polymerase-lambda-knockout-hela-cell-line-ab264754'>ab264754</a>)

Lane 3:

A549 cell lysate at 20 µg

Predicted band size: 63 kDa

Observed band size: 75 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7519(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249743 is the carrier-free version of ab172974.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DNA polymerase lambda also known as pol λ or DNA pol λ is a DNA polymerase protein involved in DNA repair and replication processes. It has a molecular weight of approximately 65 kDa. DNA polymerase lambda is expressed in various tissues but shows a high concentration in testis and thymus indicating its role in germ cells and immune response. The enzyme exhibits both DNA polymerase and terminal transferase activities facilitating DNA synthesis across gaps and lesions.

Biological function summary

DNA polymerase lambda acts as a gap-filling enzyme during DNA repair processes especially in base excision repair and non-homologous end joining. This polymerase does not typically function within a larger complex but requires accessory factors to perform its repair functions. It maintains genomic stability by providing an accurate extension of DNA strands during repair ensuring the correct rejoining of DNA. Furthermore it also exhibits a lyase activity that can remove 5'-deoxyribose phosphate residues.

Pathways

DNA polymerase lambda is actively involved in the base excision repair and non-homologous end joining pathways. These pathways are important for fixing DNA single-strand breaks and double-strand breaks respectively. Pol λ works alongside proteins like XRCC1 in the short-patch base excision repair pathway and interacts with KU70/80 in non-homologous end joining reinforcing its multifaceted role in maintaining DNA integrity under varying genotoxic stress conditions.

DNA polymerase lambda is linked to conditions such as cancer and immunodeficiencies where its dysfunction can lead to genomic instability. The loss or aberrant function of DNA pol λ may result in ineffective DNA repair contributing to cancer development due to accumulated DNA damage. It can also interact with tumor suppressor p53 highlighting its involvement in pathways that prevent oncogenesis. Additionally impairments in its function can contribute to immunodeficiency disorders as proper DNA repair is essential for the development and function of the immune system.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA polymerase that functions in several pathways of DNA repair (PubMed : 11457865, PubMed : 19806195, PubMed : 20693240, PubMed : 30250067). Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PubMed : 11457865, PubMed : 19806195). Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination (PubMed : 19806195, PubMed : 20693240, PubMed : 30250067). Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities (PubMed : 10887191, PubMed : 10982892, PubMed : 12809503, PubMed : 14627824, PubMed : 15537631, PubMed : 19806195). Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity (PubMed : 11457865, PubMed : 19806195).

See full target information POLL

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNA Polymerase lambda antibody [EPR7519(2)] - BSA and Azide free

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] - BSA and Azide free (AB249743)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Cell Lines & Lysates

Primary Antibodies

Primary Antibodies

Product promise