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Rabbit Recombinant Monoclonal DNAJA1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Predicted
Not recommended
Not recommended
Predicted
Rat
Predicted
Not recommended
Not recommended
Predicted

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).

Alternative names

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Rabbit Recombinant Monoclonal DNAJA1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR7248

Purity

Tissue culture supernatant

Dissociation constant

4.2 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774), expandable thumbnail

    Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)

    Lanes 1-4: Merged signal (red and green). Green - ab126774 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab126774 Anti-DNAJA1 antibody [EPR7248] was shown to specifically react with DNAJA1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266437 (knockout cell lysate ab257925) was used. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE. ab126774 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-DNAJA1 antibody [EPR7248] (AB126774) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: DNAJA1 knockout HEK293T cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (AB216773) at 1/10000 dilution

    Predicted band size: 45 kDa

    Observed band size: 49 kDa

    This data was developed using the same antibody clone in a different buffer formulation (ab126774).

    Lanes 1-4: Merged signal (red and green). Green - ab126774 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab126774 Anti-DNAJA1 antibody [EPR7248] was shown to specifically react with DNAJA1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266437 (knockout cell lysate ab257925) was used. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE. ab126774 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774), expandable thumbnail

    Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)

    Lane 1: Wild-type DNAJA1 whole cell lysate (20 μg)
    Lane 2: DNAJA1 knockout DNAJA1 whole cell lysate (20 μg)
    Lane 3: HepG2 whole cell lysate (20 μg)
    Lane 4: Jurkat whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab126774 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab126774 was shown to recognize DNAJA1 in wild-type HAP1 samples along with additional cross-reactive bands. No band was observed when DNAJA1 knockout samples were examined. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE. Ab126774 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-DNAJA1 antibody [EPR7248] (AB126774)

    Predicted band size: 45 kDa

  • Flow Cytometry (Intracellular) - Anti-DNAJA1 antibody [EPR7248] (ab126774), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-DNAJA1 antibody [EPR7248] (ab126774)

    ab126774 at 1/10 dilution staining DNAJA1 in permeabilized Jurkat cells by intracellular flow cytometry (red). Rabbit IgG negative control (green).

  • Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774), expandable thumbnail

    Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)

    All lanes: Western blot - Anti-DNAJA1 antibody [EPR7248] (AB126774) at 1/10000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: SKBR 3 cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: Raji cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 45 kDa

  • OI-RD Scanning - Anti-DNAJA1 antibody [EPR7248] (ab126774), expandable thumbnail

    OI-RD Scanning - Anti-DNAJA1 antibody [EPR7248] (ab126774)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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