Rabbit Recombinant Monoclonal DNAJA1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Expected |
Mouse | Predicted | Not recommended | Not recommended | Predicted |
Rat | Predicted | Not recommended | Not recommended | Predicted |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as a co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).
DNAJ2, HDJ2, HSJ2, HSPF4, DNAJA1, DnaJ homolog subfamily A member 1, DnaJ protein homolog 2, HSDJ, Heat shock 40 kDa protein 4, Heat shock protein J2, Human DnaJ protein 2, HSJ-2, hDj-2
Rabbit Recombinant Monoclonal DNAJA1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ab248179 is the carrier-free version of Anti-DNAJA1 antibody [EPR7248] ab126774.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using the same antibody clone in a different buffer formulation (Anti-DNAJA1 antibody [EPR7248] ab126774).
Lanes 1-4: Merged signal (red and green). Green - Anti-DNAJA1 antibody [EPR7248] ab126774 observed at 49 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-DNAJA1 antibody [EPR7248] ab126774 Anti-DNAJA1 antibody [EPR7248] was shown to specifically react with DNAJA1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human DNAJA1 knockout HEK-293T cell line ab266437 (knockout cell lysate Human DNAJA1 knockout HEK-293T cell lysate ab257925) was used. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE. Anti-DNAJA1 antibody [EPR7248] ab126774 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-DNAJA1 antibody [EPR7248] (Anti-DNAJA1 antibody [EPR7248] ab126774) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: DNAJA1 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human DNAJA1 knockout HEK-293T cell line (Human DNAJA1 knockout HEK-293T cell line ab266437)
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 49 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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