AB157216

Anti-DNAJA2 antibody [EPR11302(B)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal DNAJA2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CPR3, HIRIP4, DNAJA2, DnaJ homolog subfamily A member 2, Cell cycle progression restoration gene 3 protein, Dnj3, HIRA-interacting protein 4, Renal carcinoma antigen NY-REN-14, Dj3

3 Images

Flow Cytometry (Intracellular) - Anti-DNAJA2 antibody [EPR11302(B)] (AB157216)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-DNAJA2 antibody [EPR11302(B)] (AB157216)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling DNAJA2 with either ab157216 at 1/10 dilution (red) or a Rabbit IgG negative control (green).

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Western blot - Anti-DNAJA2 antibody [EPR11302(B)] (AB157216)

All lanes:

Western blot - Anti-DNAJA2 antibody [EPR11302(B)] (ab157216) at 1/50000 dilution

All lanes:

Immunoprecipitation pellet from HepG2 cell lysate at 10 µg

Predicted band size: 46 kDa

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Western blot - Anti-DNAJA2 antibody [EPR11302(B)] (AB157216)

All lanes:

Western blot - Anti-DNAJA2 antibody [EPR11302(B)] (ab157216) at 1/50000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11302(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Co-chaperone of Hsc70. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed : 24318877).

See full target information DNAJA2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4484 PubMed38802340

2024

Proximal protein landscapes of the type I interferon signaling cascade reveal negative regulation by PJA2.

Applications

Unspecified application

Species

Unspecified reactive species

Samira Schiefer,Benjamin G Hale

Cell discovery 9:107 PubMed37907457

2023

Heat shock protein DNAJA2 regulates transcription-coupled repair by triggering CSB degradation via chaperone-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping Huang,Liya Gu,Guo-Min Li

eLife 6: PubMed28504929

2017

Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes.

Applications

Unspecified application

Species

Unspecified reactive species

Nadinath B Nillegoda,Antonia Stank,Duccio Malinverni,Niels Alberts,Anna Szlachcic,Alessandro Barducci,Paolo De Los Rios,Rebecca C Wade,Bernd Bukau

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNAJA2 antibody [EPR11302(B)]

Flow Cytometry (Intracellular) - Anti-DNAJA2 antibody [EPR11302(B)] (AB157216)

Western blot - Anti-DNAJA2 antibody [EPR11302(B)] (AB157216)

Western blot - Anti-DNAJA2 antibody [EPR11302(B)] (AB157216)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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