AB185553

Anti-DNAJA4 antibody [EPR8756]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal DNAJA4 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

DnaJ homolog subfamily A member 4, DNAJA4

3 Images

Flow Cytometry (Intracellular) - Anti-DNAJA4 antibody [EPR8756] (AB185553)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DNAJA4 antibody [EPR8756] (AB185553)

Intracellular flow cytometric analysis of DNAJA4in 2% paraformaldehydeHeLa cellsusing ab185553 at 1/300 dilution (pink). Secondary antibody : Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control : Rabbit monoclonal IgG (green).

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Immunoprecipitation - Anti-DNAJA4 antibody [EPR8756] (AB185553)

Western blot analysis of Immunoprecipitate from Human fetal brain lysate (lane 1) using ab185553 at 1/50 dilution. Lane 2 : PBS used instead of Human fetal brain lysates. Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution. Blocking buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-DNAJA4 antibody [EPR8756] (ab185553)

Predicted band size: 45 kDa

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Supplier Data

Western blot - Anti-DNAJA4 antibody [EPR8756] (AB185553)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-DNAJA4 antibody [EPR8756] (ab185553) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

false

Carrier free

Anti-DNAJA4 antibody [EPR8756] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8756

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/300", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DNAJA4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:697 PubMed37875476

2023

DNAJA4 suppresses epithelial-mesenchymal transition and metastasis in nasopharyngeal carcinoma via PSMD2-mediated MYH9 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Zhang,Ping Feng,Xun-Hua Zhu,Shi-Qing Zhou,Ming-Liang Ye,Xiao-Jing Yang,Sha Gong,Sheng-Yan Huang,Xi-Rong Tan,Shi-Wei He,Ying-Qing Li

Journal of dermatological science 91:256-267 PubMed29807809

2018

DNAJA4 deficiency enhances NF-kappa B-related growth arrest induced by hyperthermia in human keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Zhe Sun,Yi Ren,Yu-Jing Zhang,Yang Han,Yang Yang,Ya-Li Gao,Li-Li Zhu,Rui-Qun Qi,Hong-Duo Chen,Xing-Hua Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNAJA4 antibody [EPR8756]

Flow Cytometry (Intracellular) - Anti-DNAJA4 antibody [EPR8756] (AB185553)

Immunoprecipitation - Anti-DNAJA4 antibody [EPR8756] (AB185553)

Western blot - Anti-DNAJA4 antibody [EPR8756] (AB185553)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise