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AB154410

Anti-DNAJB12 antibody

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(2 Publications)

Rabbit Polyclonal DNAJB12 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DNAJB12 aa 50 to C-terminus.

View Alternative Names

DnaJ homolog subfamily B member 12, DNAJB12

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB12 antibody (AB154410)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB12 antibody (AB154410)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, labeling DNAJB12 using ab154410 at a 1/500 dilution.

Western blot - Anti-DNAJB12 antibody (AB154410)
  • WB

Unknown

Western blot - Anti-DNAJB12 antibody (AB154410)

10% SDS PAGE

All lanes:

Western blot - Anti-DNAJB12 antibody (ab154410) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Lane 3:

HCT116 whole cell lysate at 30 µg

Lane 4:

MCF7 whole cell lysate at 30 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DNAJB12 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NXW2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway (PubMed : 21148293, PubMed : 21150129). Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities (PubMed : 21148293). Can also act independently of HSPA8/Hsc70 : together with DNAJB14, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers (PubMed : 27916661). While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70 (PubMed : 27916661). When overexpressed, forms membranous structures together with DNAJB14 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear (PubMed : 24732912).. (Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection (PubMed : 21673190, PubMed : 24675744).
See full target information DNAJB12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:13711-13726 PubMed41064490

2025

Comprehensive Analysis and Experimental Validation of Immune-Related Biomarkers and Immune Microenvironment in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Weini Zhou,Ziyang Zeng,Xunjia Li,Mei Yang

OncoTargets and therapy 13:7641-7652 PubMed32801777

2020

HNF1A-Induced lncRNA HCG18 Facilitates Gastric Cancer Progression by Upregulating DNAJB12 via miR-152-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Ma,Lianhai Li,Fu Liu,Qi Zhao
View all publications
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Product promise

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