Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free

5 product images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free (ab240346)

  Immunohistochemical analysis of paraffin-embedded human testis tissue sections labeling DNAJB6 with Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995).

  Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free (ab240346)

  Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue sections labeling DNAJB6 with Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995).

  Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunoprecipitation - Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free (ab240346)

  DNAJB6 was immunoprecipitated from Jurkat (Human acute T cell leukemia) whole cell extract with Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995 at 1/150 dilution. Western blot was performed from the immunoprecipitate using Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

  Lane 1: Jurkat (Human acute T cell leukemia) whole cell extract (Input) 10 µg.
  Lane 2: Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995 IP in Jurkat (Human acute T cell leukemia) whole cell extract.
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995 in Jurkat (Human acute T cell leukemia) whole cell extract.

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995).

  All lanes: Immunoprecipitation - Anti-DNAJB6 antibody [EPR17122] - N-terminal (Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995)

  Predicted band size: 36 kDa

• 

  Immunocytochemistry/ Immunofluorescence - Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free (ab240346)

  Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y cell line labeling DNAJB6 with Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit was used as the secondary at a 1/500 dilution. Counterstained using Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary at a 1/500 dilution.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995).

• 

  Flow Cytometry (Intracellular) - Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free (ab240346)

  Intracellular Flow Cytometry analysis of DNAJB6 expression in 2% paraformaldehyde-fixed Jurkat (Human acute T cell leukemia) cells cells using Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995 at 1/500 dilution (red), a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DNAJB6 antibody [EPR17122] - N-terminal ab198995).