Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free (AB240346)

Intracellular Flow Cytometry analysis of DNAJB6 expression in 2% paraformaldehyde-fixed Jurkat (Human acute T cell leukemia) cells cells using ab198995 at 1/500 dilution (red), a Rabbit monoclonal IgG (ab172730) IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198995).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free (AB240346)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y cell line labeling DNAJB6 with ab198995 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077 AlexaFluor®488 Goat anti-Rabbit was used as the secondary at a 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution with ab150120 AlexaFluor®594 Goat anti-Mouse secondary at a 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198995).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free (AB240346)

Immunohistochemical analysis of paraffin-embedded human testis tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198995).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

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Immunoprecipitation - Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free (AB240346)

DNAJB6 was immunoprecipitated from Jurkat (Human acute T cell leukemia) whole cell extract with ab198995 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab198995 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Jurkat (Human acute T cell leukemia) whole cell extract (Input) 10 µg.
Lane 2 : ab198995 IP in Jurkat (Human acute T cell leukemia) whole cell extract.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab198995 in Jurkat (Human acute T cell leukemia) whole cell extract.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198995).

All lanes:

Immunoprecipitation - Anti-DNAJB6 antibody [EPR17122] - N-terminal (<a href='/en-us/products/primary-antibodies/dnajb6-antibody-epr17122-n-terminal-ab198995'>ab198995</a>)

Predicted band size: 36 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] - BSA and Azide free (AB240346)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198995).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.