AB118282

Anti-DNAJB9 antibody

Name: Anti-DNAJB9 antibody (ab118282) | Abcam
Brand: Abcam Limited
SKU: AB118282
Price: 470 USD
Availability: InStock

(1 Review)

(4 Publications)

Goat Polyclonal DNAJB9 antibody. Suitable for ICC, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DNAJB9 aa 50-100.

View Alternative Names

MDG1, UNQ743/PRO1471, DNAJB9, DnaJ homolog subfamily B member 9, Endoplasmic reticulum DNA J domain-containing protein 4, Microvascular endothelial differentiation gene 1 protein, ER-resident protein ERdj4, ERdj4, Mdg-1

4 Images

Flow Cytometry (Intracellular) - Anti-DNAJB9 antibody (AB118282)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DNAJB9 antibody (AB118282)

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) labeling DNAJB9 with ab118282. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (1 μg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor^® 488 secondary antibody.

Supplier Data

Immunocytochemistry - Anti-DNAJB9 antibody (AB118282)

Immunocytochemistry analysis of paraformaldehyde fixed U2OS cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL).

Supplier Data

Immunocytochemistry - Anti-DNAJB9 antibody (AB118282)

Immunocytochemistry analysis of paraformaldehyde fixed A431 cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL).

Supplier Data

Western blot - Anti-DNAJB9 antibody (AB118282)

Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

All lanes:

Western blot - Anti-DNAJB9 antibody (ab118282) at 1 µg/mL

Lane 1:

Human liver lysate (in RIPA buffer) at 35 µg

Lane 2:

Human kidney lysate (in RIPA buffer) at 35 µg

Lane 3:

Mouse liver lysate (in RIPA buffer) at 35 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC, WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human DNAJB9 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UBS3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab118282 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity). J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins (PubMed : 18400946). In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP : HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1 (By similarity). Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production (By similarity).

See full target information DNAJB9

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 32:995-1009 PubMed37332476

2023

Deficiency of miR-409-3p improves myocardial neovascularization and function through modulation of DNAJB9/p38 MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Furkan Bestepe,Colette Fritsche,Kartik Lakhotiya,Carolyn E Niosi,George F Ghanem,Gregory L Martin,Ruma Pal-Ghosh,Dakota Becker-Greene,James Weston,Ivana Hollan,Ivar Risnes,Stein Erik Rynning,Liv Heidi Solheim,Mark W Feinberg,Robert M Blanton,Basak Icli

Virus research 296:198350 PubMed33626380

2021

The ORF8 protein of SARS-CoV-2 induced endoplasmic reticulum stress and mediated immune evasion by antagonizing production of interferon beta.

Applications

Unspecified application

Species

Unspecified reactive species

Farooq Rashid,Emmanuel Enoch Dzakah,Haiying Wang,Shixing Tang

Molecular cancer research : MCR 18:847-858 PubMed32102897

2020

Heat Shock Protein 47 Maintains Cancer Cell Growth by Inhibiting the Unfolded Protein Response Transducer IRE1α.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Yoneda,Kaori Sakai-Sawada,Kenjiro Minomi,Yasuaki Tamura

Oncotarget 8:9267-9279 PubMed28030827

2016

Reducing Smad3/ATF4 was essential for Sirt1 inhibiting ER stress-induced apoptosis in mice brown adipose tissue.

Applications

Species

Unspecified reactive species

Zhenjiang Liu,Huihui Gu,Lu Gan,Yatao Xu,Fei Feng,Muhammad Saeed,Chao Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNAJB9 antibody

Flow Cytometry (Intracellular) - Anti-DNAJB9 antibody (AB118282)

Immunocytochemistry - Anti-DNAJB9 antibody (AB118282)

Immunocytochemistry - Anti-DNAJB9 antibody (AB118282)

Western blot - Anti-DNAJB9 antibody (AB118282)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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