Goat Polyclonal DNAJB9 antibody. Suitable for ICC, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DNAJB9 aa 50-100.
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Publications
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- Molecular therapy. Nucleic acids 32:995-10092023Deficiency of miR-409-3p improves myocardial neovascularization and function through modulation of DNAJB9/p38 MAPK signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFurkan Bestepe,Colette Fritsche,Kartik Lakhotiya,Carolyn E Niosi,George F Ghanem,Gregory L Martin,Ruma Pal-Ghosh,Dakota Becker-Greene,James Weston,Ivana Hollan,Ivar Risnes,Stein Erik Rynning,Liv Heidi Solheim,Mark W Feinberg,Robert M Blanton,Basak IcliPubMed 37332476
- Virus research 296:1983502021The ORF8 protein of SARS-CoV-2 induced endoplasmic reticulum stress and mediated immune evasion by antagonizing production of interferon beta.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFarooq Rashid,Emmanuel Enoch Dzakah,Haiying Wang,Shixing TangPubMed 33626380
- Molecular cancer research : MCR 18:847-8582020Heat Shock Protein 47 Maintains Cancer Cell Growth by Inhibiting the Unfolded Protein Response Transducer IRE1α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkihiro Yoneda et. al.PubMed 32102897
- Oncotarget 8:9267-92792016Reducing Smad3/ATF4 was essential for Sirt1 inhibiting ER stress-induced apoptosis in mice brown adipose tissue.Applications:WBReactive species:Unspecified reactive speciesZhenjiang Liu,Huihui Gu,Lu Gan,Yatao Xu,Fei Feng,Muhammad Saeed,Chao SunPubMed 28030827
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DNAJB9 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBS3
Reactivity data
Select an application| ICC | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Pig, Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Pig, Chimpanzee | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Pig, Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity). J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins (PubMed:18400946). In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP: HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1 (By similarity). Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production (By similarity).
Alternative names
MDG1, UNQ743/PRO1471, DNAJB9, DnaJ homolog subfamily B member 9, Endoplasmic reticulum DNA J domain-containing protein 4, Microvascular endothelial differentiation gene 1 protein, ER-resident protein ERdj4, ERdj4, Mdg-1
Recommended products
Goat Polyclonal DNAJB9 antibody. Suitable for ICC, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DNAJB9 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DNAJB9 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBS3
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab118282 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunocytochemistry - Anti-DNAJB9 antibody (ab118282)
Immunocytochemistry analysis of paraformaldehyde fixed U2OS cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).
Western blot - Anti-DNAJB9 antibody (ab118282)
Primary incubation 1 hour at room temperature. Detected by chemiluminescence.
All lanes: Western blot - Anti-DNAJB9 antibody (ab118282) at 1 µg/mL
Lane 1: Human liver lysate (in RIPA buffer) at 35 µg
Lane 2: Human kidney lysate (in RIPA buffer) at 35 µg
Lane 3: Mouse liver lysate (in RIPA buffer) at 35 µg
Predicted band size: 26 kDa
Immunocytochemistry - Anti-DNAJB9 antibody (ab118282)
Immunocytochemistry analysis of paraformaldehyde fixed A431 cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).
Flow Cytometry (Intracellular) - Anti-DNAJB9 antibody (ab118282)
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) labeling DNAJB9 with ab118282. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (1 μg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
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