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Rabbit Recombinant Monoclonal DNAJC11 antibody. C-terminal. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Western blot - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (AB183518), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (AB183518), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (AB183518), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/90
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Isoform 1. Required for mitochondrial inner membrane organization. Seems to function through its association with the MICOS complex and the mitochondrial outer membrane sorting assembly machinery (SAM) complex.

Alternative names

DnaJ homolog subfamily C member 11, DNAJC11

Recommended products

Rabbit Recombinant Monoclonal DNAJC11 antibody. C-terminal. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15065(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (ab183518), expandable thumbnail

    Western blot - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (ab183518)

    All lanes: Western blot - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (ab183518) at 1/20000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: HepG2 cell lysate at 20 µg

    Lane 3: SH-SY5Y cell lysate at 20 µg

    Lane 4: A549 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (ab183518), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (ab183518)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DNAJC11 with ab183518 at 1/250 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (ab183518), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (ab183518)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling DNAJC11with ab183518 at 1/90 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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