AB231318

Anti-DNAJC2/ZRF1 antibody

Name: Anti-DNAJC2/ZRF1 antibody (ab231318) | Abcam
Brand: Abcam Limited
SKU: AB231318
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal DNAJC2/ZRF1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Dnajc2 aa 350 to C-terminus.

View Alternative Names

MPHOSPH11, MPP11, ZRF1, DNAJC2, DnaJ homolog subfamily C member 2, M-phase phosphoprotein 11, Zuotin-related factor 1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC2/ZRF1 antibody (AB231318)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC2/ZRF1 antibody (AB231318)

Paraffin-embedded mouse stomach tissue stained for DNAJC2/ZRF1 using ab231318 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC2/ZRF1 antibody (AB231318)

Paraffin-embedded mouse testis tissue stained for DNAJC2/ZRF1 using ab231318 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC2/ZRF1 antibody (AB231318)

Paraffin-embedded mouse liver tissue stained for DNAJC2/ZRF1 using ab231318 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC2/ZRF1 antibody (AB231318)

Paraffin-embedded mouse kidney tissue stained for DNAJC2/ZRF1 using ab231318 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-DNAJC2/ZRF1 antibody (AB231318)

All lanes:

Western blot - Anti-DNAJC2/ZRF1 antibody (ab231318) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 3:

Mouse testis lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 71 kDa

false

Supplier Data

Western blot - Anti-DNAJC2/ZRF1 antibody (AB231318)

All lanes:

Western blot - Anti-DNAJC2/ZRF1 antibody (ab231318) at 1 µg/mL

All lanes:

Recombinant mouse DNAJC2 protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 38 kDa,43 kDa,71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Dnajc2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54103

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231318 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone : component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state : specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation.

See full target information DNAJC2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

European journal of nutrition 62:1845-1857 PubMed36853380

2023

Long-term dietary nitrate supplementation slows the progression of established atherosclerosis in ApoE mice fed a high fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Kevin D Croft,Trevor A Mori,Tracey A Gaspari,Barbara K Kemp-Harper,Natalie C Ward

Molecular medicine reports 24: PubMed34165158

2021

DNAJC2 is reversely regulated by miR‑627‑3p, promoting the proliferation of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongfu Liu,Juan Li,Huali Zhao,Xiaohui Liu,Xinying Ye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNAJC2/ZRF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC2/ZRF1 antibody (AB231318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC2/ZRF1 antibody (AB231318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC2/ZRF1 antibody (AB231318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC2/ZRF1 antibody (AB231318)

Western blot - Anti-DNAJC2/ZRF1 antibody (AB231318)

Western blot - Anti-DNAJC2/ZRF1 antibody (AB231318)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise