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AB86434

Anti-DNAJC21 antibody

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(1 Publication)

Rabbit Polyclonal DNAJC21 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DNAJC21 aa 400-450.

View Alternative Names

DNAJA5, DNAJC21, DnaJ homolog subfamily C member 21, DnaJ homolog subfamily A member 5, Protein GS3

2 Images
Immunoprecipitation - Anti-DNAJC21 antibody (AB86434)
  • IP

Unknown

Immunoprecipitation - Anti-DNAJC21 antibody (AB86434)

Detection of Human DNAJC21 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86434 at 3 ug/mg lysate. Subsequent Western blot analysis was performed using ab86434 at 1 ug/ml.

All lanes:

Immunoprecipitation - Anti-DNAJC21 antibody (ab86434)

Predicted band size: 62 kDa

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Western blot - Anti-DNAJC21 antibody (AB86434)
  • WB

Unknown

Western blot - Anti-DNAJC21 antibody (AB86434)

All lanes:

Western blot - Anti-DNAJC21 antibody (ab86434) at 0.04 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 5 µg

Lane 4:

293T cell lysate at 50 µg

Predicted band size: 62 kDa

Observed band size: 70 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DNAJC21 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

Q5F1R6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a co-chaperone for HSP70. May play a role in ribosomal RNA (rRNA) biogenesis, possibly in the maturation of the 60S subunit. Binds the precursor 45S rRNA.
See full target information DNAJC21
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of human genetics 99:115-24 PubMed27346687

2016

DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome to Corruption in 60S Ribosome Subunit Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Hemanth Tummala,Amanda J Walne,Mike Williams,Nicholas Bockett,Laura Collopy,Shirleny Cardoso,Alicia Ellison,Rob Wynn,Thierry Leblanc,Jude Fitzgibbon,David P Kelsell,David A van Heel,Elspeth Payne,Vincent Plagnol,Inderjeet Dokal,Tom Vulliamy
View all publications
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Product promise

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