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Rabbit Polyclonal DNAJC3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DnaJ homolog subfamily C member 3 aa 250-500.

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Images

Western blot - Anti-DNAJC3 antibody (AB154714), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human DnaJ homolog subfamily C member 3 aa 250-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q13217

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted
Cow
Predicted
Pig
Predicted

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow, Pig

Dilution info

-

Notes

-

Target data

Function

Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (By similarity). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.

Alternative names

Recommended products

Rabbit Polyclonal DNAJC3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DnaJ homolog subfamily C member 3 aa 250-500.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human DnaJ homolog subfamily C member 3 aa 250-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q13217
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Western blot - Anti-DNAJC3 antibody (ab154714), expandable thumbnail

    Western blot - Anti-DNAJC3 antibody (ab154714)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-DNAJC3 antibody (ab154714) at 1/1000 dilution

    Lane 1: A431 whole cell lysate at 30 µg

    Lane 2: H1299 whole cell lysate at 30 µg

    Lane 3: HeLa whole cell lysate at 30 µg

    Lane 4: MOLT4 whole cell lysate at 30 µg

    Lane 5: Raji whole cell lysate at 30 µg

    Predicted band size: 58 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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