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AB262876

Anti-DNAJC6 antibody

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Rabbit Polyclonal DNAJC6 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DNAJC6 aa 450 to C-terminus.

View Alternative Names

KIAA0473, DNAJC6, Auxilin, DnaJ homolog subfamily C member 6

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC6 antibody (AB262876)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC6 antibody (AB262876)

Paraffin-embedded Human cerebral cortex tissue stained for DNAJC6 using ab262876 at 1/2500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-DNAJC6 antibody (AB262876)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DNAJC6 antibody (AB262876)

PFA-fixed, Triton X-100 permeabilized human U-2OS cells stained for DNAJC6 (green) using ab262876 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC6 antibody (AB262876)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC6 antibody (AB262876)

Paraffin-embedded Human pancreas tissue stained for DNAJC6 using ab262876 at 1/2500 dilution in immunohistochemical analysis.

Western blot - Anti-DNAJC6 antibody (AB262876)
  • WB

Supplier Data

Western blot - Anti-DNAJC6 antibody (AB262876)

All lanes:

Western blot - Anti-DNAJC6 antibody (ab262876) at 0.4 µg/mL

All lanes:

Human bone marrow erythroleukemia cell line

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DNAJC6 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75061

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a protein phosphatase and/or a lipid phosphatase. Co-chaperone that recruits HSPA8/HSC70 to clathrin-coated vesicles (CCVs) and promotes the ATP-dependent dissociation of clathrin from CCVs and participates in clathrin-mediated endocytosis of synaptic vesicles and their recycling and also in intracellular trafficking (PubMed : 18489706). Firstly, binds tightly to the clathrin cages, at a ratio of one DNAJC6 per clathrin triskelion. The HSPA8 : ATP complex then binds to the clathrin-auxilin cage, initially at a ratio of one HSPA8 per triskelion leading to ATP hydrolysis stimulation and causing a conformational change in the HSPA8. This cycle is repeated three times to drive to a complex containing the clathrin-auxilin cage associated to three HSPA8 : ADP complex. The ATP hydrolysis of the third HSPA8 : ATP complex leads to a concerted dismantling of the cage into component triskelia. Then, dissociates from the released triskelia and be recycled to initiate another cycle of HSPA8's recruitment. Also acts during the early steps of clathrin-coated vesicle (CCV) formation through its interaction with the GTP bound form of DNM1 (By similarity).
See full target information DNAJC6
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Product promise

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