Rabbit Recombinant Monoclonal DNAJC7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--western-blot-img116407.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)")
![Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--immunocytochemistry-immunofluorescence-img73418.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)")
![Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--immunoprecipitation-img72029.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68288.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)")
![Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--flow-cytometry-intracellular-img75875.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Version
Target data
Function
Acts as co-chaperone regulating the molecular chaperones HSP70 and HSP90 in folding of steroid receptors, such as the glucocorticoid receptor and the progesterone receptor. Proposed to act as a recycling chaperone by facilitating the return of chaperone substrates to early stages of chaperoning if further folding is required. In vitro, induces ATP-independent dissociation of HSP90 but not of HSP70 from the chaperone-substrate complexes. Recruits NR1I3 to the cytoplasm (By similarity).
Alternative names
DnaJ homolog subfamily C member 7, Tetratricopeptide repeat protein 2, TPR repeat protein 2, TPR2, TTC2, DNAJC7
Recommended products
Rabbit Recombinant Monoclonal DNAJC7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR13349
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250129 is the carrier-free version of Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--western-blot-img116407.jpg "Western blot - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)")
Western blot - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)
This data was developed using Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 56 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--immunocytochemistry-immunofluorescence-img73418.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)")
Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)
This data was developed using Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling DNAJC7 with Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830 at 1/100 dilution.
![Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--immunoprecipitation-img72029.jpg "Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)")
Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)
This data was developed using Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) immunoprecipitated using Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830)
Predicted band size: 56 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68288.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)
This data was developed using Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling DNAJC7 with Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--flow-cytometry-intracellular-img75875.jpg "Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)")
Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)
This data was developed using Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling DNAJC7 with Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134361.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)
This data was developed using Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DNAJC7 with Anti-DNAJC7 antibody [EPR13349] - N-terminal ab179830 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-bsa-and-azide-free-ab250129--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134361.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (ab250129)")
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