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AB250129

Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal DNAJC7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TPR2, TTC2, DNAJC7, DnaJ homolog subfamily C member 7, Tetratricopeptide repeat protein 2, TPR repeat protein 2

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)

This data was developed using ab179830, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells labeling DNAJC7 with ab179830 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)

This data was developed using ab179830, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling DNAJC7 with ab179830 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)

This data was developed using ab179830, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DNAJC7 with ab179830 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)

This data was developed using ab179830, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling DNAJC7 with ab179830 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)
  • IP

Supplier Data

Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)

This data was developed using ab179830, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab179830 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (<a href='/en-us/products/primary-antibodies/dnajc7-antibody-epr13349-n-terminal-ab179830'>ab179830</a>)

Predicted band size: 56 kDa

false

Western blot - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)
  • WB

Supplier Data

Western blot - Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free (AB250129)

This data was developed using ab179830, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (<a href='/en-us/products/primary-antibodies/dnajc7-antibody-epr13349-n-terminal-ab179830'>ab179830</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 56 kDa

false

  • Unconjugated

    Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 660 APC

    APC Anti-DNAJC7 antibody [EPR13349] (N-terminal)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • HRP

    HRP Anti-DNAJC7 antibody [EPR13349] (N-terminal)

  • 578 PE

    PE Anti-DNAJC7 antibody [EPR13349] (N-terminal)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13349

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250129 is the carrier-free version of ab179830.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a co-chaperone regulating the molecular chaperones HSP70 and HSP90 in folding of steroid receptors, such as the glucocorticoid receptor and the progesterone receptor. Proposed to act as a recycling chaperone by facilitating the return of chaperone substrates to early stages of chaperoning if further folding is required. In vitro, induces ATP-independent dissociation of HSP90 but not of HSP70 from the chaperone-substrate complexes. Recruits NR1I3 to the cytoplasm (By similarity).
See full target information DNAJC7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:12868-12884 PubMed32580945

2020

Adipose mesenchymal stem cell-derived extracellular vesicles containing microRNA-26a-5p target TLR4 and protect against diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yurui Duan,Qingyang Luo,Yun Wang,Yali Ma,Fang Chen,Xiaoguang Zhu,Jun Shi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com