Rabbit Recombinant Monoclonal DNAJC7 antibody. N-terminal. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.
Images
![Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--western-blot-img116407.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)")
![Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--immunocytochemistry-immunofluorescence-img73418.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)")
![Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--immunoprecipitation-img72029.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68288.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)")
![Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--flow-cytometry-intracellular-img75875.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)")
Publications
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- Cell reports 40:1110862022Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Gao,Jinge Gu,Hong Zhang,Kai Jiang,Linlin Tang,Ren Liu,Li Zhang,Pengfei Zhang,Cong Liu,Bin Dai,Jie SongPubMed 35858576
- Cells 11:2022Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengyao Qin,Yu Xin,Yong Bian,Xuan Yang,Tao Xi,Jing XiongPubMed 35053380
- Nature neuroscience 22:1966-19742019Exome sequencing in amyotrophic lateral sclerosis implicates a novel gene, DNAJC7, encoding a heat-shock protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSali M K Farhan,Daniel P Howrigan,Liam E Abbott,Joseph R Klim,Simon D Topp,Andrea E Byrnes,Claire Churchhouse,Hemali Phatnani,Bradley N Smith,Evadnie Rampersaud,Gang Wu,Joanne Wuu,Aleksey Shatunov,Alfredo Iacoangeli,Ahmad Al Khleifat,Daniel A Mordes,Sulagna Ghosh,Kevin Eggan,Rosa Rademakers,Jacob L McCauley,Rebecca Schüle,Stephan Züchner,Michael Benatar,J Paul Taylor,Michael Nalls,Marc Gotkine,Pamela J Shaw,Karen E Morrison,Ammar Al-Chalabi,Bryan Traynor,Christopher E Shaw,David B Goldstein,Matthew B Harms,Mark J Daly,Benjamin M NealePubMed 31768050
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Acts as a co-chaperone regulating the molecular chaperones HSP70 and HSP90 in folding of steroid receptors, such as the glucocorticoid receptor and the progesterone receptor. Proposed to act as a recycling chaperone by facilitating the return of chaperone substrates to early stages of chaperoning if further folding is required. In vitro, induces ATP-independent dissociation of HSP90 but not of HSP70 from the chaperone-substrate complexes. Recruits NR1I3 to the cytoplasm (By similarity).
Alternative names
TPR2, TTC2, DNAJC7, DnaJ homolog subfamily C member 7, Tetratricopeptide repeat protein 2, TPR repeat protein 2
Recommended products
Rabbit Recombinant Monoclonal DNAJC7 antibody. N-terminal. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13349
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--western-blot-img116407.jpg "Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)")
Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
All lanes: Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 56 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--immunocytochemistry-immunofluorescence-img73418.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)")
Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
Immunofluorescent analysis of HepG2 cells labeling DNAJC7 with ab179830 at 1/100 dilution.
![Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--immunoprecipitation-img72029.jpg "Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)")
Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab179830 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
Predicted band size: 56 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68288.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling DNAJC7 with ab179830 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--flow-cytometry-intracellular-img75875.jpg "Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)")
Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling DNAJC7 with ab179830 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134361.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DNAJC7 with ab179830 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830), expandable thumbnail](https://content.abcam.com/products/images/dnajc7-antibody-epr13349-n-terminal-ab179830--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134361.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)")
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