JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB179830

Anti-DNAJC7 antibody [EPR13349] - N-terminal

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Recombinant Monoclonal DNAJC7 antibody. N-terminal. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

TPR2, TTC2, DNAJC7, DnaJ homolog subfamily C member 7, Tetratricopeptide repeat protein 2, TPR repeat protein 2

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Immunofluorescent analysis of HepG2 cells labeling DNAJC7 with ab179830 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling DNAJC7 with ab179830 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DNAJC7 with ab179830 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling DNAJC7 with ab179830 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)
  • IP

Supplier Data

Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab179830 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)

Predicted band size: 56 kDa

false

Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)
  • WB

Supplier Data

Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (AB179830)

All lanes:

Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 56 kDa

false

  • Carrier free

    Anti-DNAJC7 antibody [EPR13349] - BSA and Azide free

  • 660 APC

    APC Anti-DNAJC7 antibody [EPR13349] (N-terminal)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-DNAJC7 antibody [EPR13349] - N-terminal

  • HRP

    HRP Anti-DNAJC7 antibody [EPR13349] (N-terminal)

  • 578 PE

    PE Anti-DNAJC7 antibody [EPR13349] (N-terminal)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13349

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB21685

Anti-GRP78 BiP antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (AB21685)

  • 5
  • 71
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB236592

Anti-BCG1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-BCG1 antibody (AB236592)

  • 0
  • 0
Primary Antibodies

AB188317

Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (AB188317)

  • 5
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Acts as a co-chaperone regulating the molecular chaperones HSP70 and HSP90 in folding of steroid receptors, such as the glucocorticoid receptor and the progesterone receptor. Proposed to act as a recycling chaperone by facilitating the return of chaperone substrates to early stages of chaperoning if further folding is required. In vitro, induces ATP-independent dissociation of HSP90 but not of HSP70 from the chaperone-substrate complexes. Recruits NR1I3 to the cytoplasm (By similarity).
See full target information DNAJC7
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111086 PubMed35858576

2022

Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Gao,Jinge Gu,Hong Zhang,Kai Jiang,Linlin Tang,Ren Liu,Li Zhang,Pengfei Zhang,Cong Liu,Bin Dai,Jie Song

Cell death discovery 8:208 PubMed35436989

2022

GBP2 facilitates the progression of glioma via regulation of KIF22/EGFR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yeqing Ren,Biao Yang,Geng Guo,Jianping Zhang,Yanqi Sun,Dong Liu,Shihao Guo,Yongqiang Wu,Xiaogang Wang,Shule Wang,Wenju Zhang,Xiaolong Guo,Xuepeng Li,Ren Li,Jianhang He,Zihan Zhou

Cells 11: PubMed35053380

2022

Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyao Qin,Yu Xin,Yong Bian,Xuan Yang,Tao Xi,Jing Xiong

Nature neuroscience 22:1966-1974 PubMed31768050

2019

Exome sequencing in amyotrophic lateral sclerosis implicates a novel gene, DNAJC7, encoding a heat-shock protein.

Applications

Unspecified application

Species

Unspecified reactive species

Sali M K Farhan,Daniel P Howrigan,Liam E Abbott,Joseph R Klim,Simon D Topp,Andrea E Byrnes,Claire Churchhouse,Hemali Phatnani,Bradley N Smith,Evadnie Rampersaud,Gang Wu,Joanne Wuu,Aleksey Shatunov,Alfredo Iacoangeli,Ahmad Al Khleifat,Daniel A Mordes,Sulagna Ghosh,Kevin Eggan,Rosa Rademakers,Jacob L McCauley,Rebecca Schüle,Stephan Züchner,Michael Benatar,J Paul Taylor,Michael Nalls,Marc Gotkine,Pamela J Shaw,Karen E Morrison,Ammar Al-Chalabi,Bryan Traynor,Christopher E Shaw,David B Goldstein,Matthew B Harms,Mark J Daly,Benjamin M Neale
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com