Rabbit Recombinant Monoclonal DNAJC9 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (AB249352), expandable thumbnail](https://content.abcam.com/products/images/dnajc9-antibody-epr9856-bsa-and-azide-free-ab249352--western-blot-img165781.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (AB249352)")
![Immunoprecipitation - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (AB249352), expandable thumbnail](https://content.abcam.com/products/images/dnajc9-antibody-epr9856-bsa-and-azide-free-ab249352--immunoprecipitation-img159815.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (AB249352)")
![Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (AB249352), expandable thumbnail](https://content.abcam.com/products/images/dnajc9-antibody-epr9856-bsa-and-azide-free-ab249352--western-blot-img108189.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (AB249352)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
| Mouse | Predicted | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Acts as a dual histone chaperone and heat shock co-chaperone (PubMed:33857403). As a histone chaperone, forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers; and may thereby assist MCM2 in histone H3-H4 heterodimer recognition and facilitate the assembly of histones into nucleosomes (PubMed:33857403). May also act as a histone co-chaperone together with TONSL (PubMed:33857403). May recruit histone chaperones ASF1A, NASP and SPT2 to histone H3-H4 heterodimers (PubMed:33857403). Also plays a role as co-chaperone of the HSP70 family of molecular chaperone proteins, such as HSPA1A, HSPA1B and HSPA8 (PubMed:17182002, PubMed:33857403). As a co-chaperone, may play a role in the recruitment of HSP70-type molecular chaperone machinery to histone H3-H4 substrates, thereby maintaining the histone structural integrity (PubMed:33857403). Exhibits activity to assemble histones onto DNA in vitro (PubMed:33857403).
Alternative names
DnaJ homolog subfamily C member 9, HDJC9, DnaJ protein SB73, DNAJC9
Recommended products
Rabbit Recombinant Monoclonal DNAJC9 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9856
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249352 is the carrier-free version of Anti-DNAJC9 antibody [EPR9856] ab166612.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352), expandable thumbnail](https://content.abcam.com/products/images/dnajc9-antibody-epr9856-bsa-and-azide-free-ab249352--western-blot-img165781.jpg "Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352)")
Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352)
False colour image of Western blot: Anti-DNAJC9 antibody [EPR9856] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-DNAJC9 antibody [EPR9856] ab166612 was shown to bind specifically to DNAJC9. A band was observed at 35 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in DNAJC9 CRISPR-Cas9 edited cell line Human DNAJC9 knockout HEK-293T cell line ab266364 (CRISPR-Cas9 edited cell lysate Human DNAJC9 knockout HEK-293T cell lysate ab257926). The band observed in the CRISPR-Cas9 edited lysate lane below 35 kDa is likely to represent a truncated form of DNAJC9. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and DNAJC9 CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-DNAJC9 antibody [EPR9856] (Anti-DNAJC9 antibody [EPR9856] ab166612) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: DNAJC9 CRISPR-Cas9 edited HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human DNAJC9 knockout HEK-293T cell line (Human DNAJC9 knockout HEK-293T cell line ab266364)
Lane 3: PC-3 cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 35 kDa
![Immunoprecipitation - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352), expandable thumbnail](https://content.abcam.com/products/images/dnajc9-antibody-epr9856-bsa-and-azide-free-ab249352--immunoprecipitation-img159815.jpg "Immunoprecipitation - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352)")
Immunoprecipitation - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352)
This data was developed using Anti-DNAJC9 antibody [EPR9856] ab166612, the same antibody clone in a different buffer formulation.Immunoprecipitation. Anti-DNAJC9 antibody [EPR9856] ab166612 at 1/1000 staining DNAJC9 in 293T cell lysate immunoprecipitated using Anti-DNAJC9 antibody [EPR9856] ab166612 at 1/10. Predicted band size : 30 kDa.
All lanes: Immunoprecipitation - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352)
Predicted band size: 30 kDa
![Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352), expandable thumbnail](https://content.abcam.com/products/images/dnajc9-antibody-epr9856-bsa-and-azide-free-ab249352--western-blot-img108189.jpg "Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352)")
Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352)
This data was developed using Anti-DNAJC9 antibody [EPR9856] ab166612, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-DNAJC9 antibody [EPR9856] - BSA and Azide free (ab249352) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: U87-MG cell lysate at 10 µg
Lane 3: Raji cell lysate at 10 µg
Lane 4: SH-SY5Y cell lysate at 10 µg
Lane 5: 293T cell lysate at 10 µg
Lane 6: Jurkat cell lysate at 10 µg
SecondaryAll lanes: HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
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