Anti-DnaK antibody [8E2/2]

• WB

Supplier Data

Western blot - Anti-DnaK antibody [8E2/2] (AB69617)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-DnaK antibody [8E2/2] (ab69617)

Lane 1:

DnaK active recombinant protein

Lane 2:

E.coli cell lysate.

Predicted band size: 70 kDa

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• WB

Unknown

Western blot - Anti-DnaK antibody [8E2/2] (AB69617)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-DnaK antibody [8E2/2] (ab69617) at 0.1 µg/mL

All lanes:

E.Coli DnaK protein

Predicted band size: 70 kDa

Observed band size: 70 kDa

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• WB

CiteAb

Western blot - Anti-DnaK antibody [8E2/2] (AB69617)

DnaK western blot using anti-DnaK antibody [8E2/2] ab69617. Publication image and figure legend from Yang, B., Wang, S., et al., 2018, Front Microbiol, PubMed 29520261.

ab69617 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab69617 please see the product overview.

Effect of OI-29 on E. coli O157 : H7 motility. Representative images of swimming motility (A), growth radius after 10 h at 30°C on motility agar (B), growth in LB medium (C), and representative transmission electron micrographs (D; scale bar, 1 μm) of E. coli O157 : H7 wild-type, ΔOI-29 mutant, and complemented strain. The average number of flagella per cell, as estimated from 500 cells per strain, is 1.21 for wild-type, 0.25 for ΔOI-29 mutant, and 1.37 for the complemented strain. (E) Strains grown to exponential phase were analyzed by qRT-PCR for fliC and motA, using 16S rRNA as internal control. (F) Strains were also analyzed by immunoblot for FliC, using DnaK as loading control. Bands were quantified by densitometry and normalized to DnaK. (B,E,F) Data are mean ± SD, n = 3. ∗∗P ≤ 0.01; ∗∗∗P ≤ 0.001 by Student’s t-test.

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