Mouse Monoclonal DNAK antibody. Suitable for WB and reacts with Recombinant full length protein - Escherichia coli, Escherichia coli samples. Cited in 37 publications. Immunogen corresponding to Full Length Protein corresponding to Escherichia coli K-12 dnaK.
Images
![Western blot - Anti-DnaK antibody [8E2/2] (AB69617), expandable thumbnail](https://content.abcam.com/products/images/dnak-antibody-8e2-2-ab69617--western-blot-img134558.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DnaK antibody [8E2/2] (AB69617)")
![Western blot - Anti-DnaK antibody [8E2/2] (AB69617), expandable thumbnail](https://content.abcam.com/products/images/dnak-antibody-8e2-2-ab69617--western-blot-img134559.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DnaK antibody [8E2/2] (AB69617)")
![Western blot - Anti-DnaK antibody [8E2/2] (AB69617), expandable thumbnail](https://content.abcam.com/products/images/dnak-antibody-8e2-2-ab69617--western-blot-img442122.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DnaK antibody [8E2/2] (AB69617)")
Publications
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- Nature ecology & evolution 7:2067-20792023Selection of a de novo gene that can promote survival of Escherichia coli by modulating protein homeostasis pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIdan Frumkin,Michael T LaubPubMed 37945946
- Nature communications 14:72272023Enterohaemorrhagic E. coli utilizes host- and microbiota-derived L-malate as a signaling molecule for intestinal colonization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Liu,Lingyan Jiang,Yutao Liu,Hongmin Sun,Jun Yan,Chenbo Kang,Bin YangPubMed 37945607
- Nature communications 13:62102022Promiscuity of response regulators for thioredoxin steers bacterial virulence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJu-Sim Kim,Alexandra Born,James Karl A Till,Lin Liu,Sashi Kant,Morkos A Henen,Beat Vögeli,Andrés Vázquez-TorresPubMed 36266276
- Brain, behavior, and immunity 107:110-1232022Microbial molecule ingress promotes neuroinflammation and brain CCR5 expression in persons with HIV-associated neurocognitive disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam G Branton,Jason P Fernandes,Nazanin Mohammadzadeh,Mathew A L Doan,Jon D Laman,Benjamin B Gelman,Zahra Fagrouch,Ivanela Kondova,Petra Mooij,Gerrit Koopman,Christopher PowerPubMed 36202168
- Cell reports 39:1106142022Elucidation of a complete mechanical signaling and virulence activation pathway in enterohemorrhagic Escherichia coli.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Feng,Bin Yang,Yongchang Xu,Yingying Xiong,Fang Wang,Bin Liu,Wen Yang,Ting Yao,Lei WangPubMed 35385749
- PLoS pathogens 18:e10104252022Intracellular Salmonella Paratyphi A is motile and differs in the expression of flagella-chemotaxis, SPI-1 and carbon utilization pathways in comparison to intracellular S. Typhimurium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelit Cohen,Claire Hoede,Felix Scharte,Charles Coluzzi,Emiliano Cohen,Inna Shomer,Ludovic Mallet,Sébastien Holbert,Remy Felix Serre,Thomas Schiex,Isabelle Virlogeux-Payant,Guntram A Grassl,Michael Hensel,Hélène Chiapello,Ohad Gal-MorPubMed 35381053
- Proceedings of the National Academy of Sciences of the United States of America 119:e21167761192022Mechanistic insights into the subversion of the linear ubiquitin chain assembly complex by the E3 ligase IpaH1.4 of .Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianping Liu,Yaru Wang,Danni Wang,Yingli Wang,Xiaolong Xu,Yuchao Zhang,Ying Li,Mingfang Zhang,Xinyu Gong,Yubin Tang,Liqiang Shen,Miao Li,Lifeng PanPubMed 35294289
- Frontiers in microbiology 12:8257432022Arginine GlcNAcylation and Activity Regulation of PhoP by a Type III Secretion System Effector in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Xue,Yuxuan Huang,Hua Zhang,Jiaqingzi Hu,Xing Pan,Ting Peng,Jun Lv,Kun Meng,Shan LiPubMed 35126337
- Microorganisms 9:2021Combined Therapy with microRNA-Expressing and Irradiation in Melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWonsuck Yoon,Yongsung Park,Seunghyun Kim,Yongkeun Park,Chul Yong KimPubMed 34835533
- Scientific reports 11:156632021Structure of the master regulator Rns reveals an inhibitor of enterotoxigenic Escherichia coli virulence regulons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles R Midgett,Kacey Marie Talbot,Jessica L Day,George P Munson,F Jon KullPubMed 34341412
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Full Length Protein corresponding to Escherichia coli K-12 dnaK. The exact immunogen used to generate this antibody is proprietary information. Database link P0A6Y8
Reactivity data
Select an application| WB | |
|---|---|
| Escherichia coli | Tested |
| Recombinant full length protein - Escherichia coli | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Escherichia coli, Escherichia coli | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.
Alternative names
groP, grpF, seg, b0014, JW0013, dnaK, Chaperone protein DnaK, HSP70, Heat shock 70 kDa protein, Heat shock protein 70
Recommended products
Mouse Monoclonal DNAK antibody. Suitable for WB and reacts with Recombinant full length protein - Escherichia coli, Escherichia coli samples. Cited in 37 publications. Immunogen corresponding to Full Length Protein corresponding to Escherichia coli K-12 dnaK.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Full Length Protein corresponding to Escherichia coli K-12 dnaK. The exact immunogen used to generate this antibody is proprietary information. Database link P0A6Y8
- Clone number
- 8E2/2
- Purity
- Tissue culture supernatant
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Western blot - Anti-DnaK antibody [8E2/2] (ab69617), expandable thumbnail](https://content.abcam.com/products/images/dnak-antibody-8e2-2-ab69617--western-blot-img134558.jpg "Western blot - Anti-DnaK antibody [8E2/2] (ab69617)")
Western blot - Anti-DnaK antibody [8E2/2] (ab69617)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-DnaK antibody [8E2/2] (ab69617)
Lane 1: DnaK active recombinant protein
Lane 2: E.coli cell lysate.
Predicted band size: 70 kDa
![Western blot - Anti-DnaK antibody [8E2/2] (ab69617), expandable thumbnail](https://content.abcam.com/products/images/dnak-antibody-8e2-2-ab69617--western-blot-img134559.jpg "Western blot - Anti-DnaK antibody [8E2/2] (ab69617)")
Western blot - Anti-DnaK antibody [8E2/2] (ab69617)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-DnaK antibody [8E2/2] (ab69617) at 0.1 µg/mL
All lanes: E.Coli DnaK protein
Predicted band size: 70 kDa
Observed band size: 70 kDa
![Western blot - Anti-DnaK antibody [8E2/2] (ab69617), expandable thumbnail](https://content.abcam.com/products/images/dnak-antibody-8e2-2-ab69617--western-blot-img442122.jpg "Western blot - Anti-DnaK antibody [8E2/2] (ab69617)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-DnaK antibody [8E2/2] (ab69617)
DnaK western blot using anti-DnaK antibody [8E2/2] ab69617. Publication image and figure legend from Yang, B., Wang, S., et al., 2018, Front Microbiol, PubMed 29520261.
ab69617 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab69617 please see the product overview.
Effect of OI-29 on E. coli O157:H7 motility. Representative images of swimming motility (A), growth radius after 10 h at 30°C on motility agar (B), growth in LB medium (C), and representative transmission electron micrographs (D; scale bar, 1 μm) of E. coli O157:H7 wild-type, ΔOI-29 mutant, and complemented strain. The average number of flagella per cell, as estimated from 500 cells per strain, is 1.21 for wild-type, 0.25 for ΔOI-29 mutant, and 1.37 for the complemented strain. (E) Strains grown to exponential phase were analyzed by qRT-PCR for fliC and motA, using 16S rRNA as internal control. (F) Strains were also analyzed by immunoblot for FliC, using DnaK as loading control. Bands were quantified by densitometry and normalized to DnaK. (B,E,F) Data are mean ± SD, n = 3. ∗∗P ≤ 0.01; ∗∗∗P ≤ 0.001 by Student’s t-test.
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