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AB317684

Anti-DNase I antibody [EPR25941-20] - BSA and Azide free

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Rabbit Recombinant Monoclonal DNase I antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

DNL1, DRNI, DNASE1, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I

1 Images
Western blot - Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (AB317684)
  • WB

Supplier Data

Western blot - Anti-DNase I antibody [EPR25941-20] - BSA and Azide free (AB317684)

This data was developed using ab317683, the same antibody clone in a different buffer formulation.

The bands observed at higher mw could be detection of post-translational modifications (PMID : 34329318) or glycosylated forms of the protein (PMID : 11256970).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 48 seconds, lane 2 : 15 seconds

All lanes:

Western blot - Anti-DNase I antibody [EPR25941-20] (<a href='/en-us/products/primary-antibodies/dnase-i-antibody-epr25941-20-ab317683'>ab317683</a>) at 1/1000 dilution

Lane 1:

Human stomach tissue lysate at 20 µg

Lane 2:

Human pancreas tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25941-20

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB234056

DNase I Assay Kit (Fluorometric)

Functional Studies - DNase I Assay Kit (Fluorometric) (AB234056)

  • 4
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Assay Kits

AB234056

DNase I Assay Kit (Fluorometric)

Functional Studies - DNase I Assay Kit (Fluorometric) (AB234056)

  • 4
  • 1
Primary Antibodies

AB203669

Anti-DNase gamma antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase gamma antibody (AB203669)

  • 0
  • 0
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Reactivity data

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Product details

ab317684 is the carrier-free version of ab317683.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed : 11241278, PubMed : 2251263, PubMed : 2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed : 11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
See full target information DNASE1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com