Rabbit Polyclonal DNMT1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 27 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Dnmt1 aa 1-250.
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- Antioxidants (Basel, Switzerland) 12:2023Korean Red Ginseng Attenuates Particulate Matter-Induced Senescence of Skin Keratinocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyoung Ah Kang,Mei Jing Piao,Pincha Devage Sameera Madushan Fernando,Herath Mudiyanselage Udari Lakmini Herath,Joo Mi Yi,Jin Won HyunPubMed 37627511
- Cell death discovery 8:1602022Smurf2-induced degradation of SMAD2 causes inhibition of hair follicle stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBojie Lin,Dan Huang,Guanyu Lin,Yong Miao,Jin Wang,Zhexiang Fan,Zhiqi HuPubMed 35379779
- Andrologia 54:e143572022DNMT enzymes differentially alter global DNA methylation in a stage-dependent manner during spermatogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatma Uysal,Gozde Sukur,Ozgur CinarPubMed 34997784
- Journal of molecular histology 53:63-742021Embryo culture media differentially alter DNA methylating enzymes and global DNA methylation in embryos and oocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatma Uysal,Selda Kahveci,Gozde Sukur,Ozgur CinarPubMed 34741214
- Science advances 7:eabe85112021Mutation-induced DNMT1 cleavage drives neurodegenerative disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWencai Wang,Xingsen Zhao,Yanjiao Shao,Xiaoya Duan,Yaling Wang,Jialun Li,Jiwen Li,Dali Li,Xuekun Li,Jiemin WongPubMed 34516921
- Nature structural & molecular biology 28:594-6032021Dnmt1 has de novo activity targeted to transposable elements.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuck Haggerty,Helene Kretzmer,Christina Riemenschneider,Abhishek Sampath Kumar,Alexandra L Mattei,Nina Bailly,Judith Gottfreund,Pay Giesselmann,Raha Weigert,Björn Brändl,Pascal Giehr,René Buschow,Christina Galonska,Ferdinand von Meyenn,Melissa B Pappalardi,Michael T McCabe,Lars Wittler,Claudia Giesecke-Thiel,Thorsten Mielke,David Meierhofer,Bernd Timmermann,Franz-Josef Müller,Jörn Walter,Alexander MeissnerPubMed 34140676
- Nature communications 12:32432021Impact of DNA methylation on 3D genome structure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiana Buitrago,Mireia Labrador,Juan Pablo Arcon,Rafael Lema,Oscar Flores,Anna Esteve-Codina,Julie Blanc,Nuria Villegas,David Bellido,Marta Gut,Pablo D Dans,Simon C Heath,Ivo G Gut,Isabelle Brun Heath,Modesto OrozcoPubMed 34050148
- Cell stem cell 28:1549-1565.e122021Principles of signaling pathway modulation for enhancing human naive pluripotency induction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan Bayerl,Muneef Ayyash,Tom Shani,Yair Shlomo Manor,Ohad Gafni,Rada Massarwa,Yael Kalma,Alejandro Aguilera-Castrejon,Mirie Zerbib,Hadar Amir,Daoud Sheban,Shay Geula,Nofar Mor,Leehee Weinberger,Segev Naveh Tassa,Vladislav Krupalnik,Bernardo Oldak,Nir Livnat,Shadi Tarazi,Shadi Tawil,Emilie Wildschutz,Shahd Ashouokhi,Lior Lasman,Varda Rotter,Suhair Hanna,Dalit Ben-Yosef,Noa Novershtern,Sergey Viukov,Jacob H HannaPubMed 33915080
- Cell cycle (Georgetown, Tex.) :1-132021Long non-coding RNA HOTAIR knockdown alleviates gouty arthritis through miR-20b upregulation and NLRP3 downregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa-Fei Liu et. al.PubMed 33467979
- Cellular signalling 77:1098082020LINC00922 regulates epithelial-mesenchymal transition, invasive and migratory capacities in breast cancer through promoting NKD2 methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Wang,Tianfu Dong,Peishun Wang,Shuqin Li,Geng Wu,Jun Zhou,Zhiqi WangPubMed 33045317
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Dnmt1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P13864
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.2-1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).
Alternative names
AIM, CXXC9, DNMT, DNMT1, DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, MCMT, DNA MTase HsaI, M.HsaI
Recommended products
Rabbit Polyclonal DNMT1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 27 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Dnmt1 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Dnmt1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P13864
- Purification technique
- Affinity purification Immunogen
- Specificity
ab87656 is recommended for DNMT1 detection in WB.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Useful for analysis of the protein complex containing Dnmt1.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody (ab87654)
ab87654 being used to indicate the subcellular localization of Dnmt1 in mouse embryonic stem cells (E14) during cell cycle progression.
Western blot - Anti-Dnmt1 antibody (ab87654)
Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts. Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene. Biotylated Np95 was captured by streptoavidin beads. The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 (ab87654) and Pcna.
All lanes: Western blot - Anti-Dnmt1 antibody (ab87654) at 1 µg/mL
Lane 1: Tagged-NP 95 + BirA Input
Lane 2: Tagged-NP 95 + BirA Beads
Lane 3: BirA Input
Lane 4: BirA Beads
Predicted band size: 183 kDa
Observed band size: 183 kDa
Western blot - Anti-Dnmt1 antibody (ab87654)
Catalytic activity of NP95 associated Dnmt1 in HeLa cells. The presence of Dnmt1 in the NP95 complexes was confirmed by western blot.
All lanes: Western blot - Anti-Dnmt1 antibody (ab87654) at 1 µg/mL
Lane 1: Dnmt1 1+/+ (J1) (nuclear extract)
Lane 2: Dnmt1 1-/- (36-2D6) (nuclear extract)
Lane 3: Tagged-NP95 + BirA (0.1M KCl fraction)
Lane 4: BirA (0.1M KCl fraction)
Predicted band size: 183 kDa
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