AB87654

Anti-Dnmt1 antibody

Name: Anti-Dnmt1 antibody (ab87654) | Abcam
Brand: Abcam Limited
SKU: AB87654
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(31 Publications)

Rabbit Polyclonal DNMT1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 31 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Dnmt1 aa 1-250.

View Alternative Names

AIM, CXXC9, DNMT, DNMT1, DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, MCMT, DNA MTase HsaI, M.HsaI

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody (AB87654)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody (AB87654)

ab87654 being used to indicate the subcellular localization of Dnmt1 in mouse embryonic stem cells (E14) during cell cycle progression.

Unknown

Western blot - Anti-Dnmt1 antibody (AB87654)

Catalytic activity of NP95 associated Dnmt1 in HeLa cells. The presence of Dnmt1 in the NP95 complexes was confirmed by western blot.

All lanes:

Western blot - Anti-Dnmt1 antibody (ab87654) at 1 µg/mL

Lane 1:

Dnmt1 1+/+ (J1) (nuclear extract)

Lane 2:

Dnmt1 1-/- (36-2D6) (nuclear extract)

Lane 3:

Tagged-NP95 + BirA (0.1M KCl fraction)

Lane 4:

BirA (0.1M KCl fraction)

Predicted band size: 183 kDa

false

Unknown

Western blot - Anti-Dnmt1 antibody (AB87654)

Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts. Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene. Biotylated Np95 was captured by streptoavidin beads. The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 (ab87654) and Pcna.

All lanes:

Western blot - Anti-Dnmt1 antibody (ab87654) at 1 µg/mL

Lane 1:

Tagged-NP 95 + BirA Input

Lane 2:

Tagged-NP 95 + BirA Beads

Lane 3:

BirA Input

Lane 4:

BirA Beads

Predicted band size: 183 kDa

Observed band size: 183 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Mouse Dnmt1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P13864

Specificity

ab87656 is recommended for DNMT1 detection in WB.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5000", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

Product details

Useful for analysis of the protein complex containing Dnmt1.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6 - 8.5 Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). Promotes tumor growth (PubMed : 24623306).

See full target information DNMT1

style

left-column

Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular histology 55:427-436 PubMed38850446

2024

Altered DNA methylation and Dnmt expression in obese uterus may cause implantation failure.

Applications

Unspecified application

Species

Unspecified reactive species

Nazlican Bozdemir,Tuba Kablan,Mehmet Ozgen Altintas,Gozde Sukur,Ozgur Cinar,Fatma Uysal

Histochemistry and cell biology 162:215-224 PubMed38627305

2024

Antioxidant supplementation may effect DNA methylation patterns, apoptosis, and ROS levels in developing mouse embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Uysal,Gozde Sukur,Nazlican Bozdemir,Ozgur Cinar

Genesis (New York, N.Y. : 2000) 62:e23579 PubMed37985411

2023

Unveiling the impact of DNA methylation machinery: Dnmt1 and Dnmt3a in orchestrating oocyte development and cellular homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Uysal,Gozde Sukur,Nazlican Bozdemir,Ozgur Cinar

Antioxidants (Basel, Switzerland) 12: PubMed37627511

2023

Korean Red Ginseng Attenuates Particulate Matter-Induced Senescence of Skin Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoung Ah Kang,Mei Jing Piao,Pincha Devage Sameera Madushan Fernando,Herath Mudiyanselage Udari Lakmini Herath,Joo Mi Yi,Jin Won Hyun

Cell death discovery 8:160 PubMed35379779

2022

Smurf2-induced degradation of SMAD2 causes inhibition of hair follicle stem cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Bojie Lin,Dan Huang,Guanyu Lin,Yong Miao,Jin Wang,Zhexiang Fan,Zhiqi Hu

Andrologia 54:e14357 PubMed34997784

2022

DNMT enzymes differentially alter global DNA methylation in a stage-dependent manner during spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Uysal,Gozde Sukur,Ozgur Cinar

Journal of molecular histology 53:63-74 PubMed34741214

2021

Embryo culture media differentially alter DNA methylating enzymes and global DNA methylation in embryos and oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Uysal,Selda Kahveci,Gozde Sukur,Ozgur Cinar

Science advances 7:eabe8511 PubMed34516921

2021

Mutation-induced DNMT1 cleavage drives neurodegenerative disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wencai Wang,Xingsen Zhao,Yanjiao Shao,Xiaoya Duan,Yaling Wang,Jialun Li,Jiwen Li,Dali Li,Xuekun Li,Jiemin Wong

Nature structural & molecular biology 28:594-603 PubMed34140676

2021

Dnmt1 has de novo activity targeted to transposable elements.

Applications

Unspecified application

Species

Unspecified reactive species

Chuck Haggerty,Helene Kretzmer,Christina Riemenschneider,Abhishek Sampath Kumar,Alexandra L Mattei,Nina Bailly,Judith Gottfreund,Pay Giesselmann,Raha Weigert,Björn Brändl,Pascal Giehr,René Buschow,Christina Galonska,Ferdinand von Meyenn,Melissa B Pappalardi,Michael T McCabe,Lars Wittler,Claudia Giesecke-Thiel,Thorsten Mielke,David Meierhofer,Bernd Timmermann,Franz-Josef Müller,Jörn Walter,Alexander Meissner

Nature communications 12:3243 PubMed34050148

2021

Impact of DNA methylation on 3D genome structure.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Buitrago,Mireia Labrador,Juan Pablo Arcon,Rafael Lema,Oscar Flores,Anna Esteve-Codina,Julie Blanc,Nuria Villegas,David Bellido,Marta Gut,Pablo D Dans,Simon C Heath,Ivo G Gut,Isabelle Brun Heath,Modesto Orozco

View all publications

style

left-column

style

left-column

style

right-column