Rabbit Recombinant Monoclonal DNMT1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (AB222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunocytochemistry-immunofluorescence-img126156.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (AB222228)")
![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (AB222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunocytochemistry-immunofluorescence-img68785.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (AB222228)")
![Flow Cytometry (Intracellular) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (AB222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--flow-cytometry-intracellular-img13736.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (AB222228)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (AB222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57117.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (AB222228)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (AB222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123494.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (AB222228)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Expected |
| Mouse | Expected | Predicted | Predicted | Predicted |
| Rat | Expected | Expected | Expected | Tested |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
8 products for Alternative Version
Target data
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).
Alternative names
AIM, CXXC9, DNMT, DNMT1, DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, MCMT, DNA MTase HsaI, M.HsaI
Recommended products
Rabbit Recombinant Monoclonal DNMT1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18453
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab222228 is the carrier-free version of Anti-Dnmt1 antibody [EPR18453] ab188453.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunocytochemistry-immunofluorescence-img126156.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)
Anti-Dnmt1 antibody [EPR18453] ab188453 staining Dnmt1 in wild-type HAP1 cells (top panel) and DNMT1 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-Dnmt1 antibody [EPR18453] ab188453 at 1μg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR18453] ab188453).
![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunocytochemistry-immunofluorescence-img68785.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling Dnmt1 with Anti-Dnmt1 antibody [EPR18453] ab188453 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Neuro-2a cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Dnmt1 antibody [EPR18453] ab188453 at 1/2000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR18453] ab188453).
![Flow Cytometry (Intracellular) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--flow-cytometry-intracellular-img13736.jpg "Flow Cytometry (Intracellular) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
Flow Cytometry (Intracellular) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling with purified Anti-Dnmt1 antibody [EPR18453] ab188453 at 1/100 dilution (10ug/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR18453] ab188453).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57117.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Dnmt1 with Anti-Dnmt1 antibody [EPR18453] ab188453 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on human placenta is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR18453] ab188453).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123494.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Dnmt1 with Anti-Dnmt1 antibody [EPR18453] ab188453 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on mouse stomach is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR18453] ab188453).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunocytochemistry-immunofluorescence-img71625.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Dnmt1 with Anti-Dnmt1 antibody [EPR18453] ab188453 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Dnmt1 antibody [EPR18453] ab188453 at 1/2000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR18453] ab188453).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105163.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Dnmt1 with Anti-Dnmt1 antibody [EPR18453] ab188453 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on rat stomach is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR18453] ab188453).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunocytochemistry-immunofluorescence-img15010.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Dnmt1 with Anti-Dnmt1 antibody [EPR18453] ab188453 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HEK-293 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Dnmt1 antibody [EPR18453] ab188453 at 1/2000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR18453] ab188453).
![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunocytochemistry-immunofluorescence-img71625.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105163.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228), expandable thumbnail](https://content.abcam.com/products/images/dnmt1-antibody-epr18453-bsa-and-azide-free-ab222228--immunocytochemistry-immunofluorescence-img15010.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] - BSA and Azide free (ab222228)")
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