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Rabbit Recombinant Monoclonal DNMT1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (AB191382), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (AB191382), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (AB191382), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (AB191382), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (AB191382), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

5 products for Alternative Version

Target data

Function

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).

Alternative names

AIM, CXXC9, DNMT, DNMT1, DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, MCMT, DNA MTase HsaI, M.HsaI

Recommended products

Rabbit Recombinant Monoclonal DNMT1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR3521(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab191382 is the carrier-free version of Anti-Dnmt1 antibody [EPR3521(2)] ab134148.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382), expandable thumbnail

    Western blot - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: DNMT1 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: HEK293 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-Dnmt1 antibody [EPR3521(2)] ab134148 observed at 183 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-Dnmt1 antibody [EPR3521(2)] ab134148 was shown to recognize DNMT1 when DNMT1 knockout samples were used, along with additional cross-reactive bands. Wild-type and DNMT1 knockout samples were subjected to SDS-PAGE. Anti-Dnmt1 antibody [EPR3521(2)] ab134148 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR3521(2)] ab134148).

    All lanes: Western blot - Anti-Dnmt1 antibody [EPR3521(2)] (Anti-Dnmt1 antibody [EPR3521(2)] ab134148)

    Predicted band size: 183 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382)

    Anti-Dnmt1 antibody [EPR3521(2)] ab134148 staining Dnmt1 in wild-type HAP1 cells (top panel) and DNMT1 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-Dnmt1 antibody [EPR3521(2)] ab134148 at 1/250 dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR3521(2)] ab134148).

  • Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382)

    Clone EPR3521(2) (ab191382) has been successfully conjugated by Abcam. This image was generated using Anti-Dnmt1 antibody [EPR3521(2)] (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Anti-Dnmt1 antibody [EPR3521(2)] ab197272 for protocol details.

    Alexa Fluor® 488 Anti-Dnmt1 antibody [EPR3521(2)] ab197272 staining Dnmt1 in wild-type HAP1 cells (top panel) and Dnmt1 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 488 Anti-Dnmt1 antibody [EPR3521(2)] ab197272 at 1/500 (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Dnmt1 with purified Anti-Dnmt1 antibody [EPR3521(2)] ab134148 at 1/700. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR3521(2)] ab134148).

  • Flow Cytometry (Intracellular) - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Dnmt1 (red) with unpurified Anti-Dnmt1 antibody [EPR3521(2)] ab134148 at a 1/20 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR3521(2)] ab134148).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Dnmt1 with unpurified Anti-Dnmt1 antibody [EPR3521(2)] ab134148 at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR3521(2)] ab134148).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)] - BSA and Azide free (ab191382)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Dnmt1 with unpurified Anti-Dnmt1 antibody [EPR3521(2)] ab134148 at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt1 antibody [EPR3521(2)] ab134148).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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