JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB220175

Anti-Dnmt2 antibody [ABM1H70]

Be the first to review this product! Submit a review

|

(3 Publications)

Mouse Monoclonal Dnmt2 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRDMT1.

View Alternative Names

DNMT2, TRDMT1, tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, DNA methyltransferase homolog HsaIIP, PuMet, Dnmt2, DNA MTase homolog HsaIIP, M.HsaIIP

2 Images
Flow Cytometry - Anti-Dnmt2 antibody [ABM1H70] (AB220175)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Dnmt2 antibody [ABM1H70] (AB220175)

Flow cytometric analysis of HeLa cells (4% paraformaldehyde-fixed and permeabilized) labeling Dnmt2 with ab220175 at 0.5 μg per 106 cells, followed by goat anti mouse PE conjugated secondary detection (red). Isotype control : green.

Western blot - Anti-Dnmt2 antibody [ABM1H70] (AB220175)
  • WB

Supplier Data

Western blot - Anti-Dnmt2 antibody [ABM1H70] (AB220175)

All lanes:

Western blot - Anti-Dnmt2 antibody [ABM1H70] (ab220175) at 4 µg/mL

Lane 1:

Jurkat cell lysate

Lane 2:

mouse embryonic liver lysate

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ABM1H70

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human TRDMT1. The exact immunogen used to generate this antibody is proprietary information.

O14717

style
left-column

Alternative Products

Primary Antibodies

AB244461

Anti-Dnmt2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt2 antibody (AB244461)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB188453

Anti-Dnmt1 antibody [EPR18453]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] (AB188453)

  • 5
  • 7
Primary Antibodies

AB76149

Anti-YB1 antibody [EP2708Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EP2708Y] (AB76149)

  • 5
  • 3
Primary Antibodies

AB121633

Anti-NSUN5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NSUN5 antibody (AB121633)

  • 4
  • 1
Primary Antibodies

AB272616

Anti-NSUN3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN3 antibody (AB272616)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5-1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4-6 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4-6 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp) (PubMed : 16424344). Has higher activity on tRNA(Asp) modified with queuosine at position 34 (PubMed : 30093495).
See full target information TRDMT1
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer gene therapy 30:149-162 PubMed36123390

2022

YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Yu,Congcong Zhang,Yikun Wang,Xiaoting Tian,Yayou Miao,Fanyu Meng,Lifang Ma,Xiao Zhang,Jinjing Xia

Cancer management and research 13:7989-8002 PubMed34707405

2021

Comprehensive Analysis of the Prognostic Value and Immune Infiltrates of the Three-m5C Signature in Colon Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qishun Geng,Qian Wei,Zhibo Shen,Yuanyuan Zheng,Longhao Wang,Wenhua Xue,Lifeng Li,Jie Zhao

Frontiers in immunology 11:1932 PubMed32973796

2020

Cathelicidin and Calprotectin Are Disparately Altered in Murine Models of Inflammatory Arthritis and Airway Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Mahadevappa Hemshekhar,Hadeesha Piyadasa,Dina Mostafa,Leola N Y Chow,Andrew J Halayko,Neeloffer Mookherjee
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com