Rabbit Recombinant Monoclonal Dnmt2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (AB308121), expandable thumbnail](https://content.abcam.com/products/images/dnmt2-antibody-epr24232-63-bsa-and-azide-free-ab308121--western-blot-img408746.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (AB308121)")
![Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (AB308121), expandable thumbnail](https://content.abcam.com/products/images/dnmt2-antibody-epr24232-63-bsa-and-azide-free-ab308121--flow-cytometry-intracellular-img408745.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (AB308121)")
![Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (AB308121), expandable thumbnail](https://content.abcam.com/products/images/dnmt2-antibody-epr24232-63-bsa-and-azide-free-ab308121--flow-cytometry-intracellular-img408744.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (AB308121)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IHC-P | ICC/IF | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp) (PubMed:16424344). Has higher activity on tRNA(Asp) modified with queuosine at position 34 (PubMed:30093495).
Alternative names
DNMT2, TRDMT1, tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, DNA methyltransferase homolog HsaIIP, PuMet, Dnmt2, DNA MTase homolog HsaIIP, M.HsaIIP
Recommended products
Rabbit Recombinant Monoclonal Dnmt2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24232-63
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (ab308121), expandable thumbnail](https://content.abcam.com/products/images/dnmt2-antibody-epr24232-63-bsa-and-azide-free-ab308121--western-blot-img408746.jpg "Western blot - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (ab308121)")
Western blot - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (ab308121)
This data was developed using 308120, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 103 seconds.All lanes: Western blot - Anti-Dnmt2 antibody [EPR24232-63] (Anti-Dnmt2 antibody [EPR24232-63] ab308120) at 1/1000 dilution
Lane 1: HeLa transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA specifically targeting Dnmt2 whole cell lysate at 20 µg
Lane 3: 293T whole cell lysate at 20 µg
Lane 4: MOLT-4 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 40 kDa
Exposure time: 103s
![Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (ab308121), expandable thumbnail](https://content.abcam.com/products/images/dnmt2-antibody-epr24232-63-bsa-and-azide-free-ab308121--flow-cytometry-intracellular-img408745.jpg "Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (ab308121)")
Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (ab308121)
This data was developed using Anti-Dnmt2 antibody [EPR24232-63] ab308120, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells labelling Dnmt2 with Anti-Dnmt2 antibody [EPR24232-63] ab308120 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or Anti-Dnmt2 antibody [EPR24232-63] ab308120.![Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (ab308121), expandable thumbnail](https://content.abcam.com/products/images/dnmt2-antibody-epr24232-63-bsa-and-azide-free-ab308121--flow-cytometry-intracellular-img408744.jpg "Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (ab308121)")
Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (ab308121)
This data was developed using Anti-Dnmt2 antibody [EPR24232-63] ab308120, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling Dnmt2 with Anti-Dnmt2 antibody [EPR24232-63] ab308120 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or Anti-Dnmt2 antibody [EPR24232-63] ab308120.
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