Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (AB308121)

This data was developed using ab308120, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells labelling Dnmt2 with ab308120 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab308120.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (AB308121)

This data was developed using ab308120, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling Dnmt2 with ab308120 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab308120.

• WB

Supplier Data

Western blot - Anti-Dnmt2 antibody [EPR24232-63] - BSA and Azide free (AB308121)

This data was developed using 308120, the same antibody clone in a different buffer formulation.  Blocking and diluting buffer and concentration : 5% NFDM/TBST. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 103 seconds.

All lanes:

Western blot - Anti-Dnmt2 antibody [EPR24232-63] (<a href='/en-us/products/primary-antibodies/dnmt2-antibody-epr24232-63-ab308120'>ab308120</a>) at 1/1000 dilution

Lane 1:

HeLa transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting Dnmt2 whole cell lysate at 20 µg

Lane 3:

293T whole cell lysate at 20 µg

Lane 4:

MOLT-4 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 40 kDa

false

Exposure time: 103s