Anti-Dnmt3a antibody

• WB

PubMed

Western blot - Anti-Dnmt3a antibody (AB2850)

Western blot analysis of DNMT3a in HeLa silenced cells. Total cell extracts were prepared 48 h after electroporation and analyzed by immunoblot with ab2850.

All lanes:

Western blot - Anti-Dnmt3a antibody (ab2850)

Predicted band size: 102 kDa

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Image from Nucleic Acids Res. 2014 Jan; 42(2): 804–821. Fig 5D, doi: 10.1093/nar/gkt920 Reproduced under the creative commons licence. https://creativecommons.org/licenses/by/4.0/

• WB

Unknown

Western blot - Anti-Dnmt3a antibody (AB2850)

Western blot of Dnmt3a on P19 cell nuclear extract using ab2850.

All lanes:

Western blot - Anti-Dnmt3a antibody (ab2850)

Predicted band size: 102 kDa

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• WB

CiteAb

Western blot - Anti-Dnmt3a antibody (AB2850)

Dnmt3a western blot using anti-Dnmt3a antibody ab2850. Publication image and figure legend from Bittencourt, D., Lee, B. H., et al., 2014, Epigenetics Chromatin, PubMed 25478012.

ab2850 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab2850 please see the product overview.

Characterization of mouse embryonic stem (ES) clonal cell lines expressing G9a bearing point mutations in the cage and non-cage residues of the ankyrin repeat (ANK) domain. (A)Top panel : Diagram of full-length mG9a showing amino acid sequence numbers and specific domains : E, Glu-rich; Cys, Cys-rich ring finger-like; ANK, ankyrin repeat; SET, methyltransferase; and Pre and Post, Cys-rich Pre-SET and post-SET. Bottom panel : Schematic representation of the structural basis for ankyrin repeat (green) recognition of dimethylated histone H3 Lysine 9 (gray). G9a residue numbers are shown. H3K9me2 binds in a partial hydrophobic cage composed of residues highlighted in red. Peptide binding is further specified by the interaction of non-cage G9a residues (highlighted in purple) with H3 Ser10, Thr11, Gly12 and Gly13. (B) Immunoblots (IB) were performed on the indicated proteins (with GAPDH as loading control) using whole cell extracts derived from wild-type (TT2) mouse embryonic stem cells (mESCs), G9a-null mESCs (knock-out; KO) or distinct clonal ESC lines (indicated by #1 and #2) stably expressing wild-type G9a (+WT) or G9a harboring the indicated point mutations in the ANK domain.

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