Rabbit Recombinant Monoclonal Dnmt3a antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 2 publications.
Images
![Western blot - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (AB232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--western-blot-img100895.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (AB232391)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (AB232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42070.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (AB232391)")
![Western blot - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (AB232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--western-blot-img104472.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (AB232391)")
![Flow Cytometry (Intracellular) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (AB232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--flow-cytometry-intracellular-img2880.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (AB232391)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (AB232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115364.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (AB232391)")
Publications
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- Biochemical and biophysical research communications 631:138-1452022SARS-CoV-2-induced hypomethylation of the ferritin heavy chain (FTH1) gene underlies serum hyperferritinemia in severe COVID-19 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJibran Sualeh Muhammad,Gehad ElGhazali,Jasmin Shafarin,Mohammad G Mohammad,Ameera Abu-Qiyas,Mawieh HamadPubMed 36183555
- Biomedicine & pharmacotherapy = Biomedecine & phar 128:1103492020Long non-coding RNA XIST contributes to osteoarthritis progression via miR-149-5p/DNMT3A axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunke Liu et. al.PubMed 32521454
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). |
Species Rat | Dilution info 1/2000 | Notes Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). |
Species Mouse | Dilution info 1/2000 | Notes Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
8 products for Alternative Version
Target data
Function
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:12138111, PubMed:16357870, PubMed:30478443). DNA methylation is coordinated with methylation of histones (PubMed:12138111, PubMed:16357870, PubMed:30478443). It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:12138111, PubMed:16357870, PubMed:30478443). May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity). Plays a role in paternal and maternal imprinting (By similarity). Required for methylation of most imprinted loci in germ cells (By similarity). Acts as a transcriptional corepressor for ZBTB18 (By similarity). Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity). Can actively repress transcription through the recruitment of HDAC activity (By similarity). Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity).
Alternative names
DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, Cysteine methyltransferase DNMT3A, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A
Recommended products
Rabbit Recombinant Monoclonal Dnmt3a antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18455
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232391 is the carrier-free version of Anti-Dnmt3a antibody [EPR18455] ab188470.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--western-blot-img100895.jpg "Western blot - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)")
Western blot - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Dnmt3a antibody [EPR18455] ab188470).
Lanes 1-4: Merged signal (red and green). Green - Anti-Dnmt3a antibody [EPR18455] ab188470 observed at 125 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-Dnmt3a antibody [EPR18455] ab188470 Anti-Dnmt3a antibody [EPR18455] was shown to specifically react with Dnmt3a in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human DNMT3A knockout HeLa cell line ab261793 (knockout cell lysate Human DNMT3A knockout HeLa cell lysate ab257128) was used. Wild-type and Dnmt3a knockout samples were subjected to SDS-PAGE. Anti-Dnmt3a antibody [EPR18455] ab188470 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Dnmt3a antibody [EPR18455] (Anti-Dnmt3a antibody [EPR18455] ab188470) at 1/2000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: Dnmt3a knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human DNMT3A knockout HeLa cell line (Human DNMT3A knockout HeLa cell line ab261793)
Lane 3: HEK-293 cell lysate at 20 µg
Lane 4: Daudi cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 125 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42070.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling Dnmt3a with purified Anti-Dnmt3a antibody [EPR18455] ab188470 at 1/2000 (0.409 μg/ml). Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt3a antibody [EPR18455] ab188470).![Western blot - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--western-blot-img104472.jpg "Western blot - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)")
Western blot - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Dnmt3a antibody [EPR18455] ab188470 observed at 125 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Dnmt3a antibody [EPR18455] ab188470 was shown to recognize Dnmt3a when Dnmt3a knockout samples were used, along with additional cross-reactive bands. Wild-type and Dnmt3a knockout samples were subjected to SDS-PAGE. Anti-Dnmt3a antibody [EPR18455] ab188470 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt3a antibody [EPR18455] ab188470).
All lanes: Western blot - Anti-Dnmt3a antibody [EPR18455] (Anti-Dnmt3a antibody [EPR18455] ab188470) at 1/5000 dilution
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: Dnmt3a knockout HAP1 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: Mouse brain tissue lysate at 20 µg
Predicted band size: 102 kDa
![Flow Cytometry (Intracellular) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--flow-cytometry-intracellular-img2880.jpg "Flow Cytometry (Intracellular) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)")
Flow Cytometry (Intracellular) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Dnmt3a with purified Anti-Dnmt3a antibody [EPR18455] ab188470 at 1/80 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt3a antibody [EPR18455] ab188470).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115364.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling Dnmt3a with purified Anti-Dnmt3a antibody [EPR18455] ab188470 at 1/2000 (0.409 μg/ml). Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt3a antibody [EPR18455] ab188470).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127901.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling Dnmt3a with purified Anti-Dnmt3a antibody [EPR18455] ab188470 at 1/2000 (0.409 μg/ml). Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt3a antibody [EPR18455] ab188470).![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--immunocytochemistry-immunofluorescence-img103616.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)")
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Dnmt3a with Anti-Dnmt3a antibody [EPR18455] ab188470 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Dnmt3a antibody [EPR18455] ab188470 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dnmt3a antibody [EPR18455] ab188470).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127901.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)")
![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-antibody-epr18455-bsa-and-azide-free-ab232391--immunocytochemistry-immunofluorescence-img103616.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody [EPR18455] - BSA and Azide free (ab232391)")
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