Rabbit Polyclonal Dnmt3a antibody. N-terminal. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DNMT3A.
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Publications
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- BMC pharmacology & toxicology 23:182022Dexmedetomidine reduces propofol-induced hippocampal neuron injury by modulating the miR-377-5p/Arc pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZong Chen,Yong Ding,Ying Zeng,Xue-Ping Zhang,Jian-Yan ChenPubMed 35337381
- Oncology reports 45:65-722021Methylation of the miR‑29b‑3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLihua Wang,Nv Mu,Nina QuPubMed 33416135
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DNMT3A. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6K1
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Expected | Tested | Tested | Expected |
| Rat | Expected | Tested | Expected | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:12138111, PubMed:16357870, PubMed:30478443). DNA methylation is coordinated with methylation of histones (PubMed:12138111, PubMed:16357870, PubMed:30478443). It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:12138111, PubMed:16357870, PubMed:30478443). May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity). Plays a role in paternal and maternal imprinting (By similarity). Required for methylation of most imprinted loci in germ cells (By similarity). Acts as a transcriptional corepressor for ZBTB18 (By similarity). Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity). Can actively repress transcription through the recruitment of HDAC activity (By similarity). Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity).
Alternative names
DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, Cysteine methyltransferase DNMT3A, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A
Recommended products
Rabbit Polyclonal Dnmt3a antibody. N-terminal. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DNMT3A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DNMT3A. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6K1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
5% SDS-PAGE gel.
All lanes: Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/2000 dilution
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg
Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell extracts at 30 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg
Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 102 kDa
Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab228691)
Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts with 5 μg of ab228691. Western blot was performed from the immunoprecipitate using ab228691.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG.
Lane 3: ab228691 IP in HEK-293T whole cell extract.All lanes: Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab228691)
Developed using the ECL technique.
Predicted band size: 102 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody - N-terminal (ab228691)
A431 cells (human epidermoid carcinoma cell line) cells stained for Dnmt3a (green) using ab228691 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody - N-terminal (ab228691)
Paraffin-embedded mouse ovary tissue stained for Dnmt3a using ab228691 at 1/500 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab228691)
Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract with 4 μg of ab228691. Western blot was performed form the immunoprecipitate using ab228691 at 1/2000 dilution.
Lane 1: HEK-293T nuclear lysate 50 μg
Lane 2: 4 μg of preimmune Rabbit IgG instead of ab228691 in HEK-293T nuclear extract.
Lane 3: ab228691 IP in HEK-293T nuclear extract.5% SDS-PAGE gel.
All lanes: Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab228691)
Developed using the ECL technique.
Predicted band size: 102 kDa
Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/1000 dilution
Lane 1: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Lane 2: Rat2 (Rat fibroblast cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 102 kDa
Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/1000 dilution
All lanes: Neuro2A (Mouse neuroblastoma cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 102 kDa
Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
5% SDS-PAGE gel.
All lanes: Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/500 dilution
Lane 1: Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cells extract at 30 µg
Lanes 2 - 3: Dnmt3a shRNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 102 kDa
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