Anti-Dnmt3a antibody - N-terminal

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody - N-terminal (AB228691)

Immunofluorescence analysis of 293T cells fixed in 4% paraformaldehyde at RT for 15 min labelling DNMT3A protein at nucleus using ab228691 at a 1/1000 dilution (Green). Alpha Tubulin, Cytoskeleton Marker was counterstained in red.

• IP

Supplier Data

Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (AB228691)

Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract with 4 μg of ab228691. Western blot was performed form the immunoprecipitate using ab228691 at 1/2000 dilution.
Lane 1 : HEK-293T nuclear lysate 50 μg
Lane 2 : 4 μg of preimmune Rabbit IgG instead of ab228691 in HEK-293T nuclear extract.
Lane 3 : ab228691 IP in HEK-293T nuclear extract.

5% SDS-PAGE gel.

All lanes:

Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab228691)

Predicted band size: 102 kDa

true

• IP

Supplier Data

Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (AB228691)

Immunoprecipitation of DNMT3A protein from 293T whole cell extracts using 5 µg of ab228691 or ab227725.

Western blot analysis was performed using ab228691 at a 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/2000 dilution

Lane 1:

293T whole cell extracts (input)

Lane 2:

293T whole cell extracts + Ctrl IgG (-)

Lanes 3 - 4:

293T whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG Antibody

Predicted band size: 102 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody - N-terminal (AB228691)

Paraffin-embedded mouse ovary tissue stained for Dnmt3a using ab228691 at 1/500 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody - N-terminal (AB228691)

Immunohistochemical analysis of paraffin-embedded mouse muscle and mouse heart tissue stained for DNMT3A protein at nucleus with ab228691 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

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Western blot - Anti-Dnmt3a antibody - N-terminal (AB228691)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/2000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 130 kDa

false

• WB

Supplier Data

Western blot - Anti-Dnmt3a antibody - N-terminal (AB228691)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cells extract at 30 µg

Lanes 2 - 3:

Dnmt3a shRNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-Dnmt3a antibody - N-terminal (AB228691)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/1000 dilution

Lane 1:

Wild-type 293T cell extracts at 30 µg

Lane 2:

DNMT3A knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 130 kDa

false

• WB

Supplier Data

Western blot - Anti-Dnmt3a antibody - N-terminal (AB228691)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/1000 dilution

All lanes:

Neuro2A (Mouse neuroblastoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 102 kDa

true

• WB

Supplier Data

Western blot - Anti-Dnmt3a antibody - N-terminal (AB228691)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/1000 dilution

Lane 1:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

Lane 2:

Rat2 (Rat fibroblast cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 102 kDa

true