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AB237985

Anti-Dnmt3a antibody [PCRP-DNMT3A-1E2]

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(1 Publication)

Mouse Monoclonal Dnmt3a antibody. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DNMT3A R882C.

View Alternative Names

DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, Cysteine methyltransferase DNMT3A, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [PCRP-DNMT3A-1E2] (AB237985)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody [PCRP-DNMT3A-1E2] (AB237985)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Dnmt3a with ab237985 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-Dnmt3a antibody [PCRP-DNMT3A-1E2] (AB237985)
  • Protein Array

Unknown

Protein Array - Anti-Dnmt3a antibody [PCRP-DNMT3A-1E2] (AB237985)

ab237985 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PCRP-DNMT3A-1E2

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, Protein Array

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DNMT3A R882C.

Q9Y6K1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.</p>" }, "Recombinant full length protein - Human": { "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed : 12138111, PubMed : 16357870, PubMed : 30478443). DNA methylation is coordinated with methylation of histones (PubMed : 12138111, PubMed : 16357870, PubMed : 30478443). It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed : 12138111, PubMed : 16357870, PubMed : 30478443). May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity). Plays a role in paternal and maternal imprinting (By similarity). Required for methylation of most imprinted loci in germ cells (By similarity). Acts as a transcriptional corepressor for ZBTB18 (By similarity). Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity). Can actively repress transcription through the recruitment of HDAC activity (By similarity). Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity).
See full target information DNMT3A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 12: PubMed36551172

2022

Evaluation of B7-H3 Targeted Immunotherapy in a 3D Organoid Model of Craniopharyngioma.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Tang,Caili Chen,Guoqing Wang,Yuelong Wang,Zongliang Zhang,Hexian Li,Qizhong Lu,Zeng Wang,Shasha Zhao,Chen Yang,Kunhong Zhong,Ruyuan Zhang,Liping Guo,Zhu Yuan,Chunlai Nie,Aiping Tong
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Product promise

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