Rabbit Recombinant Monoclonal Dnmt3a mutated R882H antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Transfected cell line - Human samples.
Images
![Western blot - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (AB271004), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-mutated-r882h-antibody-epr24337-44-ab271004--western-blot-img171783.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (AB271004)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (AB271004), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-mutated-r882h-antibody-epr24337-44-ab271004--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169940.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (AB271004)")
![Flow Cytometry (Intracellular) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (AB271004), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-mutated-r882h-antibody-epr24337-44-ab271004--flow-cytometry-intracellular-img175285.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (AB271004)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (AB271004), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-mutated-r882h-antibody-epr24337-44-ab271004--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172746.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (AB271004)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (AB271004), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-mutated-r882h-antibody-epr24337-44-ab271004--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165141.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (AB271004)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Predicted | Not recommended | Not recommended | Predicted | Predicted |
| Transfected cell line - Human | Tested | Not recommended | Not recommended | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:12138111, PubMed:16357870, PubMed:30478443). DNA methylation is coordinated with methylation of histones (PubMed:12138111, PubMed:16357870, PubMed:30478443). It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:12138111, PubMed:16357870, PubMed:30478443). May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity). Plays a role in paternal and maternal imprinting (By similarity). Required for methylation of most imprinted loci in germ cells (By similarity). Acts as a transcriptional corepressor for ZBTB18 (By similarity). Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity). Can actively repress transcription through the recruitment of HDAC activity (By similarity). Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity).
Alternative names
DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, Cysteine methyltransferase DNMT3A, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A
Recommended products
Rabbit Recombinant Monoclonal Dnmt3a mutated R882H antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24337-44
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-mutated-r882h-antibody-epr24337-44-ab271004--western-blot-img171783.jpg "Western blot - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004)")
Western blot - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004)
Dnmt3a (mutated R882H) Western blot staining using rabbit Anti-Dnmt3a (mutated R882H) antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 103 seconds
All lanes: Western blot - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with human Dnmt3a (R882H) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 3: HEK-293T transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 4: HEK-293T transfected with human Dnmt3a expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 135 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-mutated-r882h-antibody-epr24337-44-ab271004--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169940.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004)
Immunohistochemical analysis of paraffin-embedded HEK-293T transfected with human Dnmt3a (mutated R882H) expression vector (Image A) and HEK-293T transfected with human Dnmt3a wild type expression vector (Image B) tissue labelling Dnmt3a (mutated R882H) with ab271004 at 1/1000 (0.585 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Nuclear staining on HEK-293T cells transfected with human Dnmt3a (mutated R882H) expression vector (Image A) and no staining on HEK-293T cells transfected with human Dnmt3a wild type vector (Image B).
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
The section was incubated with ab271004 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Flow Cytometry (Intracellular) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-mutated-r882h-antibody-epr24337-44-ab271004--flow-cytometry-intracellular-img175285.jpg "Flow Cytometry (Intracellular) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004)")
Flow Cytometry (Intracellular) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with myc-tagged human Dnmt3a expression vector (Left)/ HEK-293T transfected with myc-tagged human Dnmt3a (mutated R882H) expression vector (Right) cells labelling Dnmt3a (mutated R882H) with ab271004 at 1/500 dilution (0.1 μg).
A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-mutated-r882h-antibody-epr24337-44-ab271004--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172746.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling Dnmt3a (mutated R822H) with ab271004 at 1/1000 (0.585 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with ab271004 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining on human tonsil.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004), expandable thumbnail](https://content.abcam.com/products/images/dnmt3a-mutated-r882h-antibody-epr24337-44-ab271004--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165141.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a (mutated R882H) antibody [EPR24337-44] (ab271004)
Immunohistochemical analysis of paraffin-embedded human testis tissue labelling Dnmt3a (mutated R882H) with ab271004 at 1/1000 (0.585 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with ab271004 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining on human testis.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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