AB122932

Anti-Dnmt3b antibody

Name: Anti-Dnmt3b antibody (ab122932) | Abcam
Brand: Abcam Limited
SKU: AB122932
Price: 430 USD
Availability: InStock

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(18 Publications)

Rabbit Polyclonal Dnmt3b antibody. Suitable for WB, ICC/IF, IHC-Fr and reacts with Mouse, Recombinant fragment samples. Cited in 18 publications.

View Alternative Names

DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase MmuIIIB, DNA MTase MmuIIIB, M.MmuIIIB

3 Images

Immunohistochemistry (Frozen sections) - Anti-Dnmt3b antibody (AB122932)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Dnmt3b antibody (AB122932)

Expression of Dnmt3b in mouse embyos was examined by immuno-fluorescence staining using 1/1000 ab122932.
Mouse embryos at E4.5, 5.5 and 7.0 were fixed in cold acetone and stained with ab122932 or DAPI (blue). The antibody was detected with anti-rabbit IgG conjugated with ALEXA568 (red). The ICM (the inner cell mass) and trophectoderm (T) are indicated by arrows and arrowheads, respectively. The epiblast (E), and the embryonic ectoderm (EE) and extraembryonic region (EX) are indicated by square brackets. Dnmt3b existed at E4.5-7.0, in ICM at E4.5, the epiblast at E5.5, and the embryonic ectoderm at E7.0.

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3b antibody (AB122932)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3b antibody (AB122932)

Immunocytochemistry/ Immunofluorescence analysis of wild-type (left) and Dnmt3b/3a knockout (right) mouse embryonic stem cells using ab122932 at 1/5000 dilution. DNA was stained with DAPI (bottom). Cell were fixed with 4% PFA/PBS for 30 min at 4℃.

Lab

Western blot - Anti-Dnmt3b antibody (AB122932)

All lanes:

Western blot - Anti-Dnmt3b antibody (ab122932) at 1/3000 dilution

Lane 1:

wild-type mouse embryonic stem cell lysate

Lane 2:

Dnmt3b/3a knockout mouse embryonic stem cell lysate

Predicted band size: 96 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, ICC/IF, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5000", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/2000 - 1/5000", "IHCFr-species-notes": "" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6 - 8.5 Preservative: 0.05% Sodium azide Constituents: Ammonium sulphate, PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Functions as a transcriptional corepressor by associating with ZHX1 (By similarity). Required for DUX4 silencing in somatic cells (By similarity).

See full target information Dnmt3b

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102268 PubMed39171140

2024

Bi-functional CpG-STAT3 decoy oligonucleotide triggers multilineage differentiation of acute myeloid leukemia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dongfang Wang,Damian Kaniowski,Karol Jacek,Yu-Lin Su,Chunsong Yu,Jeremy Hall,Haiqing Li,Mingye Feng,Susanta Hui,Bożena Kaminska,Vittorio DeFranciscis,Carla Lucia Esposito,Annalisa DiRuscio,Bin Zhang,Guido Marcucci,Ya-Huei Kuo,Marcin Kortylewski

Nucleic acids research 51:4791-4813 PubMed36919611

2023

GCNA is a histone binding protein required for spermatogonial stem cell maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Ribeiro,Gerry P Crossan

BMC cancer 22:605 PubMed35655145

2022

Global DNA hypomethylation of colorectal tumours detected in tissue and liquid biopsies may be related to decreased methyl-donor content.

Applications

Unspecified application

Species

Unspecified reactive species

Krisztina A Szigeti,Alexandra Kalmár,Orsolya Galamb,Gábor Valcz,Barbara K Barták,Zsófia B Nagy,Sára Zsigrai,Ildikó Felletár,Árpád V Patai,Tamás Micsik,Márton Papp,Eszter Márkus,Zsolt Tulassay,Peter Igaz,István Takács,Béla Molnár

Life science alliance 5: PubMed35288457

2022

DNA methylation-independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ding,Lukas Theo Schmitt,Melanie Brux,Duran Sürün,Martina Augsburg,Felix Lansing,Jovan Mircetic,Mirko Theis,Frank Buchholz

Nucleic acids research 50:2905-2922 PubMed35212377

2022

CMTR1 is recruited to transcription start sites and promotes ribosomal protein and histone gene expression in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shang Liang,Joana C Silva,Olga Suska,Radoslaw Lukoszek,Rajaei Almohammed,Victoria H Cowling

Oncogene 40:5416-5426 PubMed34282274

2021

Cancer cells escape p53's tumor suppression through ablation of ZDHHC1-mediated p53 palmitoylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Tang,Weiyan Peng,Yixiao Feng,Xin Le,Kang Wang,Qin Xiang,Lili Li,Yan Wang,Can Xu,Junhao Mu,Ke Xu,Ping Ji,Qian Tao,Ailong Huang,Chu-Xia Deng,Yong Lin,Tingxiu Xiang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2100849 PubMed34247447

2021

Pten Regulates Cardiomyocyte Differentiation by Modulating Non-CG Methylation via Dnmt3.

Applications

Unspecified application

Species

Unspecified reactive species

Wuming Wang,Gang Lu,Hong-Bin Liu,Zhiqiang Xiong,Ho-Duen Leung,Ruican Cao,Alan Lap-Yin Pang,Xianwei Su,Patrick Wai Nok Law,Zhiju Zhao,Zi-Jiang Chen,Wai-Yee Chan

Nature communications 12:3243 PubMed34050148

2021

Impact of DNA methylation on 3D genome structure.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Buitrago,Mireia Labrador,Juan Pablo Arcon,Rafael Lema,Oscar Flores,Anna Esteve-Codina,Julie Blanc,Nuria Villegas,David Bellido,Marta Gut,Pablo D Dans,Simon C Heath,Ivo G Gut,Isabelle Brun Heath,Modesto Orozco

Oncology reports 45:65-72 PubMed33416135

2021

Methylation of the miR‑29b‑3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Wang,Nv Mu,Nina Qu

Science advances 7: PubMed33523829

2021

Abnormal neocortex arealization and Sotos-like syndrome-associated behavior in mutant mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lichao Xu,Yue Zheng,Xuejing Li,Andi Wang,Dawei Huo,Qinglan Li,Shikang Wang,Zhiyuan Luo,Ying Liu,Fuqiang Xu,Xudong Wu,Min Wu,Yan Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Dnmt3b antibody

Immunohistochemistry (Frozen sections) - Anti-Dnmt3b antibody (AB122932)

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3b antibody (AB122932)

Western blot - Anti-Dnmt3b antibody (AB122932)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (18)

Product promise