Anti-Dnmt3b antibody - N-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3b antibody - N-terminal (AB227883)

Paraffin-embedded human lung carcinoma tissue stained for Dnmt3b using ab227883 at 1/250 dilution in immunohistochemical analysis.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3b antibody - N-terminal (AB227883)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Dnmt3b (green) using ab227883 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Red : phalloidin, a cytoskeleton marker, at 1/50 dilution.

• IP

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Immunoprecipitation - Anti-Dnmt3b antibody - N-terminal (AB227883)

Dnmt3b was immunoprecipitated from K562 (human chronic myelogenous leukemia cell line from bone marrow ) nuclear extract with 5 μg ab227883. Western blot was performed from the immunoprecipitate using ab227883. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : Control IgG IP in K562 nuclear extract.

Lane 2 : ab227883 IP in K562 nuclear extract.

All lanes:

Immunoprecipitation - Anti-Dnmt3b antibody - N-terminal (ab227883)

Predicted band size: 96 kDa

false

• WB

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Western blot - Anti-Dnmt3b antibody - N-terminal (AB227883)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Dnmt3b antibody - N-terminal (ab227883) at 1/500 dilution

Lane 1:

Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg

Lane 2:

Dnmt3b knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 96 kDa

false

• WB

Supplier Data

Western blot - Anti-Dnmt3b antibody - N-terminal (AB227883)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Dnmt3b antibody - N-terminal (ab227883) at 1/500 dilution

Lane 1:

HeLa cell extracts at 30 µg

Lane 2:

HeLa nuclear cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 95 kDa

false

• WB

Supplier Data

Western blot - Anti-Dnmt3b antibody - N-terminal (AB227883)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Dnmt3b antibody - N-terminal (ab227883) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 95 kDa

true