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AB174663

Anti-DNTTIP1/TDIF1 antibody

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(2 Publications)

Rabbit Polyclonal DNTTIP1/TDIF1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DNTTIP1 aa 250 to C-terminus.

View Alternative Names

C20orf167, TDIF1, DNTTIP1, Deoxynucleotidyltransferase terminal-interacting protein 1, Terminal deoxynucleotidyltransferase-interacting factor 1, TdIF1, TdT-interacting factor 1

2 Images
Immunoprecipitation - Anti-DNTTIP1/TDIF1 antibody (AB174663)
  • IP

Supplier Data

Immunoprecipitation - Anti-DNTTIP1/TDIF1 antibody (AB174663)

Detection of DNTTIP1/TDIF1 by Western Blot of Immunoprecipitate.<br> Immunoprecipitation of HeLa whole cell lysate using ab174663 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of DNTTIP1 with ab174663 at 1μg/ml. The lysate in lane 2 is precipitated with an IgG control antibody.

All lanes:

Immunoprecipitation - Anti-DNTTIP1/TDIF1 antibody (ab174663)

Predicted band size: 37 kDa

true

Exposure time: 30s

Western blot - Anti-DNTTIP1/TDIF1 antibody (AB174663)
  • WB

Supplier Data

Western blot - Anti-DNTTIP1/TDIF1 antibody (AB174663)

All lanes:

Western blot - Anti-DNTTIP1/TDIF1 antibody (ab174663) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 37 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DNTTIP1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H147

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Increases DNTT terminal deoxynucleotidyltransferase activity (in vitro) (PubMed : 11473582). Also acts as a transcriptional regulator, binding to the consensus sequence 5'-GNTGCATG-3' following an AT-tract. Associates with RAB20 promoter and positively regulates its transcription. Binds DNA and nucleosomes; may recruit HDAC1 complexes to nucleosomes or naked DNA.
See full target information DNTTIP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35674491

2022

METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Eriko Matsuura-Suzuki,Tadahiro Shimazu,Mari Takahashi,Kaoru Kotoshiba,Takehiro Suzuki,Kazuhiro Kashiwagi,Yoshihiro Sohtome,Mai Akakabe,Mikiko Sodeoka,Naoshi Dohmae,Takuhiro Ito,Yoichi Shinkai,Shintaro Iwasaki

Nature communications 11:3252 PubMed32591534

2020

The MiDAC histone deacetylase complex is essential for embryonic development and has a unique multivalent structure.

Applications

Unspecified application

Species

Unspecified reactive species

Robert E Turnbull,Louise Fairall,Almutasem Saleh,Emma Kelsall,Kyle L Morris,T J Ragan,Christos G Savva,Aditya Chandru,Christopher J Millard,Olga V Makarova,Corinne J Smith,Alan M Roseman,Andrew M Fry,Shaun M Cowley,John W R Schwabe
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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