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AB226797

Anti-DOCK2 antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal DOCK2 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DOCK2.

View Alternative Names

KIAA0209, DOCK2, Dedicator of cytokinesis protein 2

2 Images
Western blot - Anti-DOCK2 antibody - C-terminal (AB226797)
  • WB

Supplier Data

Western blot - Anti-DOCK2 antibody - C-terminal (AB226797)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-DOCK2 antibody - C-terminal (ab226797) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell extract at 30 µg

Lane 3:

NCI-H929 whole cell extract at 30 µg

Predicted band size: 212 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-DOCK2 antibody - C-terminal (AB226797)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DOCK2 antibody - C-terminal (AB226797)

Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for DOCK2 (green) using ab226797 at 1/400 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.

Blue : Hoechst 33342 staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human DOCK2. The exact immunogen used to generate this antibody is proprietary information.

Q92608

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.
See full target information DOCK2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Free radical biology & medicine 181:118-129 PubMed35131446

2022

RedoxiFluor: A microplate technique to quantify target-specific protein thiol redox state in relative percentage and molar terms.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmet Tuncay,Anna Noble,Matthew Guille,James N Cobley
View all publications
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Product promise

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