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AB85723

Anti-DOCK4 antibody

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(6 Publications)

Rabbit Polyclonal DOCK4 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human DOCK4 aa 1700-1800.

View Alternative Names

KIAA0716, DOCK4, Dedicator of cytokinesis protein 4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK4 antibody (AB85723)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK4 antibody (AB85723)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DOCK4 with ab85723 at 1/200 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-DOCK4 antibody (AB85723)
  • IP

Unknown

Immunoprecipitation - Anti-DOCK4 antibody (AB85723)

Detection of Human DOCK4 by Immunoprecipitation in HeLa cell lysate (1 mg for IP, 20% of IP loaded)using ab85723 at 3ug/mg lysate. Subsequent Western blot detection was performed using 0.4 ug/ml ab85723. Detection : Chemiluminescence with exposure times of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DOCK4 antibody (ab85723)

Predicted band size: 225 kDa

false

Western blot - Anti-DOCK4 antibody (AB85723)
  • WB

Unknown

Western blot - Anti-DOCK4 antibody (AB85723)

All lanes:

Western blot - Anti-DOCK4 antibody (ab85723) at 0.04 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 5 µg

Lane 4:

293T cell lysate at 50 µg

Predicted band size: 225 kDa

Observed band size: 225 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human DOCK4 aa 1700-1800. The exact immunogen used to generate this antibody is proprietary information.

Q8N1I0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "3-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP to GTP, converting inactive GDP-bound small GTPases into their active GTP-bound form (PubMed : 12628187, PubMed : 16464467). Involved in regulation of adherens junction between cells (PubMed : 12628187). Plays a role in cell migration (PubMed : 20679435).. Isoform 2. Has a higher guanine nucleotide exchange factor activity compared to other isoforms.
See full target information DOCK4
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 17:1417567 PubMed39282658

2024

Dock4 contributes to neuropathic pain by regulating spinal synaptic plasticity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaochu Fu,Hongyi Li,Zhuanxu Zhu,Wencui Li,Zhihua Ruan,Ruijie Chang,Huixia Wei,Xueqin Xu,Xunliang Xu,Yanqiong Wu

Scientific reports 14:12153 PubMed38802496

2024

HIF2α-dependent Dock4/Rac1-signaling regulates formation of adherens junctions and cell polarity in normoxia.

Applications

Unspecified application

Species

Unspecified reactive species

I Raykhel,V-P Ronkainen,J Myllyharju,A Manninen

Cancer management and research 12:9339-9349 PubMed33061623

2020

Long Noncoding RNA Promotes the Progression of Liver Cancer via Alteration of microRNA-144-3p/DOCK4 Signal.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Li,Min Wang,Hui Zhou,Shan Lu,Bo Zhang

Molecular psychiatry : PubMed31388105

2019

Autism-like social deficit generated by Dock4 deficiency is rescued by restoration of Rac1 activity and NMDA receptor function.

Applications

Unspecified application

Species

Unspecified reactive species

Daji Guo,Yinghui Peng,Laijian Wang,Xiaoyu Sun,Xiaojun Wang,Chunmei Liang,Xiaoman Yang,Shengnan Li,Junyu Xu,Wen-Cai Ye,Bin Jiang,Lei Shi

Nature 569:565-569 PubMed31019307

2019

SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Linzhang Huang,Ken L Chambliss,Xiaofei Gao,Ivan S Yuhanna,Erica Behling-Kelly,Sonia Bergaya,Mohamed Ahmed,Peter Michaely,Kate Luby-Phelps,Anza Darehshouri,Lin Xu,Edward A Fisher,Woo-Ping Ge,Chieko Mineo,Philip W Shaul

The Journal of cell biology 217:3183-3201 PubMed29934311

2018

p190 RhoGAP promotes contact inhibition in epithelial cells by repressing YAP activity.

Applications

Unspecified application

Species

Unspecified reactive species

Scott R Frank,Clemens P Köllmann,Phi Luong,Giorgio G Galli,Lihua Zou,André Bernards,Gad Getz,Raffaele A Calogero,Morten Frödin,Steen H Hansen
View all publications
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Product promise

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