Rabbit Polyclonal DOCK4 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DOCK4 aa 1700-1800.
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Publications
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- Cancer management and research 12:9339-93492020Long Noncoding RNA Promotes the Progression of Liver Cancer via Alteration of microRNA-144-3p/DOCK4 Signal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHang Li et. al.PubMed 33061623
- Molecular psychiatry :2019Autism-like social deficit generated by Dock4 deficiency is rescued by restoration of Rac1 activity and NMDA receptor function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaji Guo et. al.PubMed 31388105
- Nature 569:565-5692019SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinzhang Huang et. al.PubMed 31019307
- The Journal of cell biology 217:3183-32012018p190 RhoGAP promotes contact inhibition in epithelial cells by repressing YAP activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesScott R Frank et. al.PubMed 29934311
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DOCK4 aa 1700-1800. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N1I0
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Elephant | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant | Dilution info - | Notes - |
Target data
Function
Functions as a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP to GTP, converting inactive GDP-bound small GTPases into their active GTP-bound form (PubMed:12628187, PubMed:16464467). Involved in regulation of adherens junction between cells (PubMed:12628187). Plays a role in cell migration (PubMed:20679435). Isoform 2. Has a higher guanine nucleotide exchange factor activity compared to other isoforms.
Alternative names
KIAA0716, DOCK4, Dedicator of cytokinesis protein 4
Recommended products
Rabbit Polyclonal DOCK4 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DOCK4 aa 1700-1800.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DOCK4 aa 1700-1800. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N1I0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK4 antibody (ab85723)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DOCK4 with ab85723 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-DOCK4 antibody (ab85723)
Detection of Human DOCK4 by Immunoprecipitation in HeLa cell lysate (1 mg for IP, 20% of IP loaded)using ab85723 at 3ug/mg lysate. Subsequent Western blot detection was performed using 0.4 ug/ml ab85723. Detection:
Chemiluminescence with exposure times of 30 seconds.All lanes: Immunoprecipitation - Anti-DOCK4 antibody (ab85723)
Predicted band size: 225 kDa
Western blot - Anti-DOCK4 antibody (ab85723)
All lanes: Western blot - Anti-DOCK4 antibody (ab85723) at 0.04 µg/mL
Lane 1: HeLa cell lysate at 50 µg
Lane 2: HeLa cell lysate at 15 µg
Lane 3: HeLa cell lysate at 5 µg
Lane 4: 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 225 kDa
Observed band size: 225 kDa
Exposure time: 30s
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