AB178427

Anti-DOCK8 antibody [EPR12511-56]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal DOCK8 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Dedicator of cytokinesis protein 8, DOCK8

1 Images

Supplier Data

Western blot - Anti-DOCK8 antibody [EPR12511-56] (AB178427)

All lanes:

Western blot - Anti-DOCK8 antibody [EPR12511-56] (ab178427) at 1/1000 dilution

Lane 1:

THP1 cell line lysate at 10 µg

Lane 2:

Raji cell line lysate at 10 µg

Lane 3:

Ramos cell line lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody

Predicted band size: 239 kDa

true

Carrier free

Anti-DOCK8 antibody [EPR12511-56] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12511-56

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOCK8 also known as Dedicator of Cytokinesis 8 weighs approximately 210 kDa. It acts mechanically as a guanine nucleotide exchange factor (GEF) for small Rho GTPases which help regulate actin cytoskeleton dynamics. DOCK8 is expressed widely particularly in hematopoietic cells such as lymphocytes. It also associates with cell membranes where it facilitates signaling pathways involved in immune cell function.

Biological function summary

DOCK8 actively participates in immune system regulation by impacting the cytoskeletal rearrangements necessary for proper signaling and movement of immune cells. DOCK8 engages with proteins like DOCK2 and DOCK3 forming a complex that modulates these cellular mechanisms. Its function enhances the proper survival and immune response activities of leukocytes assisting in immune surveillance and pathogen clearance.

Pathways

Several immune-related signaling pathways involve DOCK8 especially the Rac-dependent pathways that affect actin polymerization. DOCK8 interacts closely with the CRK-like protein (CRKL) to mediate these processes. These pathways highlight the interaction between DOCK8 and other proteins critical to immune cell function positioning DOCK8 as an important regulator in immune system pathways.

Mutations in the DOCK8 gene correlate with DOCK8 deficiency a form of combined immunodeficiency characterized by eczema recurrent infections and high risk for malignancies. This disorder directly connects DOCK8 with suboptimal immune function. Furthermore DOCK8's interaction with the WASP protein plays a role in disrupting immune system health contributing to the pathophysiology of this immunodeficiency.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed : 22461490, PubMed : 28028151). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed : 28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed : 25762780).

See full target information DOCK8

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Andrology 6:345-350 PubMed29409138

2018

Treatment of semen samples with α-chymotrypsin alters the expression pattern of sperm functional proteins-a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

M K Panner Selvam,A Agarwal,R Sharma,L Samanta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DOCK8 antibody [EPR12511-56]

Western blot - Anti-DOCK8 antibody [EPR12511-56] (AB178427)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise