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AB175208

Anti-DOCK8 antibody [EPR12511]

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(6 Publications)

Rabbit Recombinant Monoclonal DOCK8 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Dedicator of cytokinesis protein 8, DOCK8

4 Images
Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] (AB175208)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] (AB175208)

Intracellular flow cytometric analysis of permeabilized THP1 cells labeling DOCK8with ab175208at 1/10 dilution (red) compared to a Rabbit IgG (green).

Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (AB175208)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (AB175208)

Immunofluorescent staining of Raji cells labeling DOCK8 with ab175208 at 1/100 dilution (green). DAPI nuclear staining (blue).

Western blot - Anti-DOCK8 antibody [EPR12511] (AB175208)
  • WB

Supplier Data

Western blot - Anti-DOCK8 antibody [EPR12511] (AB175208)

All lanes:

Western blot - Anti-DOCK8 antibody [EPR12511] (ab175208) at 1/10000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

THP1 cell lysate at 10 µg

Predicted band size: 239 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (AB175208)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (AB175208)

Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling DOCK8 with purified ab175208 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12511

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250</strong></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250</strong></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOCK8 also known as Dedicator of Cytokinesis 8 weighs approximately 210 kDa. It acts mechanically as a guanine nucleotide exchange factor (GEF) for small Rho GTPases which help regulate actin cytoskeleton dynamics. DOCK8 is expressed widely particularly in hematopoietic cells such as lymphocytes. It also associates with cell membranes where it facilitates signaling pathways involved in immune cell function.
Biological function summary

DOCK8 actively participates in immune system regulation by impacting the cytoskeletal rearrangements necessary for proper signaling and movement of immune cells. DOCK8 engages with proteins like DOCK2 and DOCK3 forming a complex that modulates these cellular mechanisms. Its function enhances the proper survival and immune response activities of leukocytes assisting in immune surveillance and pathogen clearance.

Pathways

Several immune-related signaling pathways involve DOCK8 especially the Rac-dependent pathways that affect actin polymerization. DOCK8 interacts closely with the CRK-like protein (CRKL) to mediate these processes. These pathways highlight the interaction between DOCK8 and other proteins critical to immune cell function positioning DOCK8 as an important regulator in immune system pathways.

Mutations in the DOCK8 gene correlate with DOCK8 deficiency a form of combined immunodeficiency characterized by eczema recurrent infections and high risk for malignancies. This disorder directly connects DOCK8 with suboptimal immune function. Furthermore DOCK8's interaction with the WASP protein plays a role in disrupting immune system health contributing to the pathophysiology of this immunodeficiency.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed : 22461490, PubMed : 28028151). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed : 28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed : 25762780).
See full target information DOCK8
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pediatrics 12:1420118 PubMed39108694

2024

An efficient and successful outcome after haematopoietic stem cell transplantation in a patient with an LPS-responsive beige-like anchor gene mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Cen Shen,Luying Zhang,Yan Meng,Lu Yang,Wenli He,Xiaoying Lei,Lina Zhou,Yunfei An,Ying Dou

Journal of clinical immunology 44:170 PubMed39098944

2024

A Novel Case of IFNAR1 Deficiency Identified a Common Canonical Splice Site Variant in DOCK8 in Western Polynesia: The Importance of Validating Variants of Unknown Significance in Under-Represented Ancestries.

Applications

Unspecified application

Species

Unspecified reactive species

Aimee Huynh,Paul E Gray,Anna Sullivan,Joseph Mackie,Antoine Guerin,Geetha Rao,Karrnan Pathmanandavel,Erika Della Mina,Georgina Hollway,Matthew Hobbs,Karen Enthoven,Patrick O'Young,Sam McManus,Luke H Wainwright,Megan Higgins,Fallon Noon,Melanie Wong,Paul Bastard,Qian Zhang,Jean-Laurent Casanova,Kuang-Chih Hsiao,Alberto Pinzon-Charry,Cindy S Ma,Stuart G Tangye

Clinical immunology (Orlando, Fla.) 195:36-44 PubMed30048691

2018

Diagnosis of DOCK8 deficiency using Flow cytometry Biomarkers: an Egyptian Center experience.

Applications

Unspecified application

Species

Unspecified reactive species

Safa S Meshaal,Rabab E El Hawary,Alia Eldash,Bodo Grimbacher,Nadezhda Camacho-Ordonez,Dalia S Abd Elaziz,Nermeen M Galal,Jeannette A Boutros,Shereen M Shawky,Aisha M Elmarsafy

Journal of immunology (Baltimore, Md. : 1950) 198:1887-1899 PubMed28130497

2017

Mechanisms of Impaired Neutrophil Migration by MicroRNAs in Myelodysplastic Syndromes.

Applications

Unspecified application

Species

Unspecified reactive species

Meiwan Cao,Yayoi Shikama,Hideo Kimura,Hideyoshi Noji,Kazuhiko Ikeda,Tomoyuki Ono,Kazuei Ogawa,Yasuchika Takeishi,Junko Kimura

Immunologic research 64:260-71 PubMed26659092

2015

Novel DOCK8 gene mutations lead to absence of protein expression in patients with hyper-IgE syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Qin,Yunfei An,Chaohong Liu,Junfeng Wu,Rongxin Dai,Dawei Liu,Xiaohui Li,Liping Jiang,Daoqi Wu,Xuemei Tang,Wenxia Song,Tao Wang,Xiaodong Zhao

Developmental dynamics : an official publication o 242:1160-71 PubMed23813564

2013

Canonical Wnt signaling regulates Nkx3.1 expression and luminal epithelial differentiation during prostate organogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Marianna Kruithof-de Julio,Maho Shibata,Nishita Desai,Melissa Reynon,M Vivienne Halili,Ya-Ping Hu,Sandy M Price,Cory Abate-Shen,Michael M Shen
View all publications
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