Rabbit Recombinant Monoclonal DOCK8 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182), expandable thumbnail](https://content.abcam.com/products/images/dock8-antibody-epr12511-bsa-and-azide-free-ab240182--immunocytochemistry-immunofluorescence-img111422.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)")
![Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182), expandable thumbnail](https://content.abcam.com/products/images/dock8-antibody-epr12511-bsa-and-azide-free-ab240182--immunocytochemistry-immunofluorescence-img16976.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)")
![Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182), expandable thumbnail](https://content.abcam.com/products/images/dock8-antibody-epr12511-bsa-and-azide-free-ab240182--flow-cytometry-intracellular-img14523.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Expected | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
Associated Products
Select an associated product type
1 product for Alternative Product
2 products for Alternative Version
Target data
Function
Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed:22461490, PubMed:28028151). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed:28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed:25762780).
Alternative names
Dedicator of cytokinesis protein 8, DOCK8
Recommended products
Rabbit Recombinant Monoclonal DOCK8 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12511
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240182 is the carrier-free version of Anti-DOCK8 antibody [EPR12511] ab175208.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DOCK8 also known as Dedicator of Cytokinesis 8 weighs approximately 210 kDa. It acts mechanically as a guanine nucleotide exchange factor (GEF) for small Rho GTPases which help regulate actin cytoskeleton dynamics. DOCK8 is expressed widely particularly in hematopoietic cells such as lymphocytes. It also associates with cell membranes where it facilitates signaling pathways involved in immune cell function.
- Biological function summary
DOCK8 actively participates in immune system regulation by impacting the cytoskeletal rearrangements necessary for proper signaling and movement of immune cells. DOCK8 engages with proteins like DOCK2 and DOCK3 forming a complex that modulates these cellular mechanisms. Its function enhances the proper survival and immune response activities of leukocytes assisting in immune surveillance and pathogen clearance.
- Pathways
Several immune-related signaling pathways involve DOCK8 especially the Rac-dependent pathways that affect actin polymerization. DOCK8 interacts closely with the CRK-like protein (CRKL) to mediate these processes. These pathways highlight the interaction between DOCK8 and other proteins critical to immune cell function positioning DOCK8 as an important regulator in immune system pathways.
- Associated diseases and disorders
Mutations in the DOCK8 gene correlate with DOCK8 deficiency a form of combined immunodeficiency characterized by eczema recurrent infections and high risk for malignancies. This disorder directly connects DOCK8 with suboptimal immune function. Furthermore DOCK8's interaction with the WASP protein plays a role in disrupting immune system health contributing to the pathophysiology of this immunodeficiency.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182), expandable thumbnail](https://content.abcam.com/products/images/dock8-antibody-epr12511-bsa-and-azide-free-ab240182--immunocytochemistry-immunofluorescence-img111422.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)")
Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)
Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling DOCK8 with purified Anti-DOCK8 antibody [EPR12511] ab175208 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DOCK8 antibody [EPR12511] ab175208).
![Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182), expandable thumbnail](https://content.abcam.com/products/images/dock8-antibody-epr12511-bsa-and-azide-free-ab240182--immunocytochemistry-immunofluorescence-img16976.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)")
Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)
Immunofluorescent staining of Raji cells labeling DOCK8 with Anti-DOCK8 antibody [EPR12511] ab175208 at 1/100 dilution (green). DAPI nuclear staining (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DOCK8 antibody [EPR12511] ab175208).
![Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182), expandable thumbnail](https://content.abcam.com/products/images/dock8-antibody-epr12511-bsa-and-azide-free-ab240182--flow-cytometry-intracellular-img14523.jpg "Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)")
Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (ab240182)
Intracellular flow cytometric analysis of permeabilized THP1 cells labeling DOCK8 with Anti-DOCK8 antibody [EPR12511] ab175208 at 1/10 dilution (red) compared to a Rabbit IgG (green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DOCK8 antibody [EPR12511] ab175208).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com