Rabbit Polyclonal DOCK9/Trg antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DOCK9 aa 250-400.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DOCK9 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BZ29
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation.
Alternative names
KIAA1058, ZIZ1, DOCK9, Dedicator of cytokinesis protein 9, Cdc42 guanine nucleotide exchange factor zizimin-1, Zizimin-1
Recommended products
Rabbit Polyclonal DOCK9/Trg antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DOCK9 aa 250-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DOCK9 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BZ29
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DOCK9 also known as Dedicator of Cytokinesis 9 functions as a guanine nucleotide exchange factor (GEF) specifically for the Rho family GTPases including CDC42. This protein has a molecular mass of approximately 250 kDa. DOCK9 is expressed in various tissues including high expression in the brain and lymphoid tissues. Its role primarily involves facilitating the exchange of GDP for GTP on GTPases which is important for signal transduction and cellular morphology.
- Biological function summary
DOCK9 regulates cytoskeletal organization and is part of a protein complex that influences actin polymerization. By activating CDC42 DOCK9 contributes to cellular processes such as migration proliferation and immune response. Its activity affects the modulation of actin filaments which is essential for the maintenance of cell shape and the formation of cellular protrusions such as filopodia.
- Pathways
DOCK9 has significant roles in both the PI3K-Akt signaling pathway and the Rho GTPase cycle. In these pathways DOCK9 interacts with proteins that determine cell survival growth and motility. CDC42 influences both pathways interacting with other related proteins like RAC1 which further emphasizes the importance of DOCK9 in pathways regulating cell dynamics and receptor signaling.
- Associated diseases and disorders
DOCK9 links to conditions like cancer and immunodeficiency. Alterations in its regulation or expression can lead to aberrant cell migration and proliferation contributing to tumorigenesis. Additionally DOCK9's involvement in activating CDC42 associates it with immunological dysfunctions. Changes in the CDC42 pathway can result in disrupted immune cell activity and development leading to immune-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-DOCK9/Trg antibody (ab204421)
Lane 1: Western blot - Anti-DOCK9/Trg antibody (ab204421)
Lane 2: Western blot - Anti-DOCK9/Trg antibody (ab204421) at 0.4 µg/mL
Lane 1: Molecular weight ladder
Lane 2: Caco-2 cell lysate
Predicted band size: 236 kDa, 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK9/Trg antibody (ab204421)
Paraffin embedded human placenta tissue stained for DOCK9/Trg using ab204421 at 1/1000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK9/Trg antibody (ab204421)
Paraffin embedded human cerebellum tissue stained for DOCK9/Trg using ab204421 at 1/1000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK9/Trg antibody (ab204421)
Paraffin embedded human lung tissue stained for DOCK9/Trg using ab204421 at 1/1000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK9/Trg antibody (ab204421)
Paraffin embedded human pancreas tissue stained for DOCK9/Trg using ab204421 at 1/1000 dilution in immunohistochemical analysis. Shows no positivity in exocrine glandular cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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