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AB70272

Anti-DOCK9/Trg antibody

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(1 Publication)

Rabbit Polyclonal DOCK9/Trg antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DOCK9 aa 1-50.

View Alternative Names

KIAA1058, ZIZ1, DOCK9, Dedicator of cytokinesis protein 9, Cdc42 guanine nucleotide exchange factor zizimin-1, Zizimin-1

2 Images
Immunoprecipitation - Anti-DOCK9/Trg antibody (AB70272)
  • IP

Supplier Data

Immunoprecipitation - Anti-DOCK9/Trg antibody (AB70272)

Immunoprecipitation of HeLa using 3ug/mL ab70272

Lane 1 : ab70272

All lanes:

Immunoprecipitation - Anti-DOCK9/Trg antibody (ab70272)

Predicted band size: 236 kDa

false

Western blot - Anti-DOCK9/Trg antibody (AB70272)
  • WB

Supplier Data

Western blot - Anti-DOCK9/Trg antibody (AB70272)

All lanes:

Western blot - Anti-DOCK9/Trg antibody (ab70272)

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 5 µg

Predicted band size: 236 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DOCK9 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BZ29

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/15000", "WB-species-notes": "<p></p>" }, "Opossum": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOCK9 also known as Dedicator of Cytokinesis 9 functions as a guanine nucleotide exchange factor (GEF) specifically for the Rho family GTPases including CDC42. This protein has a molecular mass of approximately 250 kDa. DOCK9 is expressed in various tissues including high expression in the brain and lymphoid tissues. Its role primarily involves facilitating the exchange of GDP for GTP on GTPases which is important for signal transduction and cellular morphology.
Biological function summary

DOCK9 regulates cytoskeletal organization and is part of a protein complex that influences actin polymerization. By activating CDC42 DOCK9 contributes to cellular processes such as migration proliferation and immune response. Its activity affects the modulation of actin filaments which is essential for the maintenance of cell shape and the formation of cellular protrusions such as filopodia.

Pathways

DOCK9 has significant roles in both the PI3K-Akt signaling pathway and the Rho GTPase cycle. In these pathways DOCK9 interacts with proteins that determine cell survival growth and motility. CDC42 influences both pathways interacting with other related proteins like RAC1 which further emphasizes the importance of DOCK9 in pathways regulating cell dynamics and receptor signaling.

DOCK9 links to conditions like cancer and immunodeficiency. Alterations in its regulation or expression can lead to aberrant cell migration and proliferation contributing to tumorigenesis. Additionally DOCK9's involvement in activating CDC42 associates it with immunological dysfunctions. Changes in the CDC42 pathway can result in disrupted immune cell activity and development leading to immune-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation.
See full target information DOCK9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 16:147 PubMed26646719

2015

The whole-genome expression analysis of peripheral blood mononuclear cells from aspirin sensitive asthmatics versus aspirin tolerant patients and healthy donors after in vitro aspirin challenge.

Applications

WB

Species

Unspecified reactive species

Joanna Wieczfinska,Dorota Kacprzak,Karolina Pospiech,Milena Sokolowska,Magdalena Nowakowska,Ewa Pniewska,Andrzej Bednarek,Izabela Kuprys-Lipinska,Piotr Kuna,Rafal Pawliczak
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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