AB254866

Anti-DOHH antibody

Name: Anti-DOHH antibody (ab254866) | Abcam
Brand: Abcam Limited
SKU: AB254866

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(1 Publication)

Rabbit Polyclonal DOHH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DOHH aa 1-150.

View Alternative Names

HLRC1, DOHH, Deoxyhypusine hydroxylase, hDOHH, Deoxyhypusine dioxygenase, Deoxyhypusine monooxygenase, HEAT-like repeat-containing protein 1

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-DOHH antibody (AB254866)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DOHH antibody (AB254866)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling DOHH using ab254866 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Formalin-fixed, paraffin-embedded human kidney tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Formalin-fixed, paraffin-embedded human hippocampus tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-DOHH antibody (AB254866)

All lanes:

Western blot - Anti-DOHH antibody (ab254866) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T cell lysate

Lane 2:

DOHH overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DOHH aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9BU89

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The deoxyhypusine hydroxylase (DOHH) enzyme also known as hDOHH catalyzes the final step in the post-translational synthesis of the unusual amino acid hypusine. DOHH modifies deoxyhypusine into hypusine by hydroxylation a reaction critical in activating the eukaryotic initiation factor 5A (eIF5A). The human DOHH protein has a mass of approximately 36 kDa and is ubiquitously expressed in various tissues reflecting its general significance in protein processing.

Biological function summary

Proteins such as DOHH play important roles in cellular growth and proliferation. DOHH functions by converting inactive eIF5A precursors into their active form which can influence mRNA translation. This enzyme does not form part of a larger complex with other proteins but its activity is essential for the hypusine modification necessary to activate eIF5A directly influencing protein synthesis.

Pathways

DOHH operates in the hypusine biosynthesis pathway which is important for cell proliferation and differentiation. DOHH acts after the enzyme deoxyhypusine synthase (DHS) introduces a deoxyhypusine residue into eIF5A illustrating the close relationship between these two proteins in this cascade. The activation of eIF5A through hypusination impacts diverse pathways that regulate cell cycle progression and apoptosis.

DOHH activity has implications in cancer and neurodegenerative diseases. Altered expression or malfunction of DOHH can influence eIF5A activity affecting cell growth and survival and therefore contributing to oncogenesis. In neurodegenerative conditions abnormal hypusine synthesis may disrupt neuronal integrity and function. The linked protein eIF5A plays a role in these diseases demonstrating the broader impact of DOHH on health outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine (PubMed : 16371467, PubMed : 16533814, PubMed : 19706422). Hypusination is unique to mature eIF-5A factor and is essential for its function (By similarity).

See full target information DOHH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 25: PubMed35419615

2022

SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Cai,Zhenhua Sun,Lili Zhang,Hongrui Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DOHH antibody

Immunocytochemistry/ Immunofluorescence - Anti-DOHH antibody (AB254866)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (AB254866)

Western blot - Anti-DOHH antibody (AB254866)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise