JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB8112

Anti-DOK1 antibody

5

(1 Review)

|

(9 Publications)

Rabbit Polyclonal DOK1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human Docking protein 1 aa 400-450.

View Alternative Names

Docking protein 1, Downstream of tyrosine kinase 1, p62(dok), pp62, DOK1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody (AB8112)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody (AB8112)

ab8112 at 2μg/ml staining DOK1 in K562 cells by ICC/IF

Western blot - Anti-DOK1 antibody (AB8112)
  • WB

Unknown

Western blot - Anti-DOK1 antibody (AB8112)

All lanes:

Western blot - Anti-DOK1 antibody (ab8112) at 1 µg/mL

Lane 1:

Jurkat cell lysates

Lane 2:

THP-1 cell lysate

Predicted band size: 52 kDa

Observed band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human Docking protein 1 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

Q99704

style
left-column

Alternative Products

Primary Antibodies

AB75741

Anti-DOK1 (phospho Y398) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK1 (phospho Y398) antibody (AB75741)

  • 0
  • 0
Primary Antibodies

AB155947

Anti-DOK1 antibody [EPR3228(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (AB155947)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB45143

Anti-CD32B antibody [EP888Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (AB45143)

  • 0
  • 0
Primary Antibodies

AB125016

Anti-Fyn antibody [EPR5500]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [EPR5500] (AB125016)

  • 4
  • 2
Primary Antibodies

AB191606

Anti-PI 3 Kinase p85 alpha antibody [EPR18702]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (AB191606)

  • 5
  • 2
Primary Antibodies

AB247258

Anti-ZAP70 antibody [YE291] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [YE291] - BSA and Azide free (AB247258)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Tyrosine kinase substrate p62DOK

Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOK1 also known as Docking protein 1 or Downstream of tyrosine kinase 1 is an adaptor protein involved in the signal transduction pathways. This protein weighs approximately 62 kDa and is primarily expressed in hematopoietic cells. While DOK1 lacks enzymatic activity it plays an important role in cellular processes by providing a scaffold for various signaling molecules. It helps to modulate downstream signaling events by interacting with receptor tyrosine kinases and other proteins through its multiple binding domains.
Biological function summary

DOK1 operates as a negative regulator in signal transduction functioning as part of a larger signaling complex. The protein associates with different cellular components forming interactions that influence cell communication and immune response. DOK1 often interacts with proteins like RasGAP and Nck which helps in mediating signals that can affect cell growth differentiation and apoptosis. Its role as a docking station for other proteins allows it to influence cellular homeostasis.

Pathways

DOK1 influences the Ras and MAP kinase signaling pathways. It inhibits the Ras pathway by binding with and recruiting RasGAP leading to the inactivation of Ras an important player in cell proliferation. Additionally DOK1 modulates the JAK/STAT pathway by affecting cytokine signaling and immune regulation. These interactions are significant for controlling cellular responses and maintaining balance in cell signaling networks.

DOK1 is implicated in conditions like leukemia and autoimmune diseases. Aberrant DOK1 expression or malfunction disrupts normal signal transduction contributing to the pathogenesis of these disorders. In leukemia altered DOK1 activity can affect its interactions with proteins such as RasGAP leading to unchecked cell growth and survival. Similarly in autoimmune diseases DOK1's regulatory effect on JAK/STAT signaling might be compromised impacting immune cell function and response.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
See full target information Docking protein 1
style
left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1021-1034 PubMed40419795

2025

Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

James R W Conway,Omkar Joshi,Jasmin Kaivola,Gautier Follain,Michalis Gounis,David Kühl,Johanna Ivaska

International journal of molecular medicine 46:1409-1422 PubMed32945349

2020

Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Sheng,Xiuqin Cao,Shuhong Chi,Jing Wu,Huihui Xing,Huiyu Liu,Zhiwei Yang

EBioMedicine 8:159-172 PubMed27428427

2016

Subcellular compartmentalization of docking protein-1 contributes to progression in colorectal cancer.

Applications

IHC

Species

Unspecified reactive species

Teresa Friedrich,Michaela Söhn,Tobias Gutting,Klaus-Peter Janssen,Hans-Michael Behrens,Christoph Röcken,Matthias P A Ebert,Elke Burgermeister

The EMBO journal 33:1928-40 PubMed24963146

2014

Dok1 and Dok2 proteins regulate natural killer cell development and function.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Celis-Gutierrez,Marilyn Boyron,Thierry Walzer,Pier Paolo Pandolfi,Stipan Jonjić,Daniel Olive,Marc Dalod,Eric Vivier,Jacques A Nunès

PLoS pathogens 10:e1004125 PubMed24809689

2014

Epstein-Barr virus down-regulates tumor suppressor DOK1 expression.

Applications

WB

Species

Unspecified reactive species

Maha Siouda,Cecilia Frecha,Rosita Accardi,Jiping Yue,Cyrille Cuenin,Henri Gruffat,Evelyne Manet,Zdenko Herceg,Bakary S Sylla,Massimo Tommasino

Journal of cell science 127:2647-58 PubMed24762811

2014

A crucial role for DOK1 in PDGF-BB-stimulated glioma cell invasion through p130Cas and Rap1 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Barrett,Ian M Evans,Antonina Frolov,Gary Britton,Caroline Pellet-Many,Maiko Yamaji,Vedanta Mehta,Rina Bandopadhyay,Ningning Li,Sebastian Brandner,Ian C Zachary,Paul Frankel

Molecular and cellular biology 32:4877-90 PubMed23028047

2012

Transcriptional regulation of the human tumor suppressor DOK1 by E2F1.

Applications

WB

Species

Human

Maha Siouda,Jiping Yue,Ruchi Shukla,Sophie Guillermier,Zdenko Herceg,Marion Creveaux,Rosita Accardi,Massimo Tommasino,Bakary S Sylla

Science signaling 4:ra59 PubMed21917715

2011

Feedback circuits monitor and adjust basal Lck-dependent events in T cell receptor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie R Schoenborn,Ying Xim Tan,Chao Zhang,Kevan M Shokat,Arthur Weiss

Molecular immunology 46:2133-9 PubMed19371953

2009

Src-like adaptor protein (SLAP) is upregulated in antigen-stimulated mast cells and acts as a negative regulator.

Applications

WB

Species

Unspecified reactive species

Seung-Kiel Park,Huihong Qiao,Michael A Beaven
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com